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Title: <b>Tissue Culture</b>
Issue Date: Aug-2005
Publisher: CSIR
Abstract: <i>In vitro </i>regeneration of the species was carried out on Murashige & Skoog’s (MS) medium using leaf and rhizome explants. On MS medium supplemented with 2mg/l NAA and 1mg/l BA, compact greenish white callus resulted from leaf explants. From leaf explants on MS + 2 mg/l 2, 4-D + 0.5mg/l BA, friable callus was obtained. The compact callus also yielded friable callus when transferred to B5 medium supplemented with 0.6mg/l 2,4-D and 0.4mg/l NAA. These calli were non-morphogenetic. Morphogenetic compact callus derived from leaf and rhizome explants differentiated shoots on MS + 1.0mg/l BA + 6mg/l KN. Good rooting response was observed when regenerated shoots were inoculated in modified white root culture medium and ¼ MS with 1.5% sucrose. Such plantlets were successfully transferred to soil after hardening, with a high rate of survival. The plants were comparable to natural population in growth and vigour [Prajapati A Hiren, Mehta R Saurabh and Subramanian RB, <i>In</i> <i>vitro </i>regeneration in <i>C u r c u l i g o</i> <i>orchioides </i>Gaertn.- An endangered medicinal herb, <i>Phytomorphology</i>, 2004, <b>54</b>(1 & 2), 85-95].
Description: 297-298
ISSN: 0975-1092 (Online); 0972-592X (Print)
Appears in Collections:NPR Vol.4(4) [July-August 2005]

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