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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Natural Products and Resources (IJNPR)
[Formerly Natural Product Radiance (NPR)] >
NPR Vol.4 [2005] >
NPR Vol.4(4) [July-August 2005] >
| Title: | Tissue Culture |
| Issue Date: | Aug-2005 |
| Publisher: | CSIR |
| Abstract: | In vitro regeneration
of the species was
carried out on Murashige & Skoog’s (MS) medium
using leaf and rhizome explants. On MS medium
supplemented with 2mg/l NAA and 1mg/l BA,
compact greenish white callus resulted from leaf
explants. From leaf explants on MS + 2 mg/l 2,
4-D + 0.5mg/l BA, friable callus was obtained.
The compact callus also yielded friable callus when
transferred to B5 medium
supplemented with
0.6mg/l 2,4-D and 0.4mg/l NAA. These calli
were non-morphogenetic. Morphogenetic
compact callus derived from leaf and rhizome
explants differentiated shoots on MS + 1.0mg/l
BA + 6mg/l KN. Good rooting response was
observed when regenerated shoots were inoculated
in modified white root culture medium and ¼
MS with 1.5% sucrose. Such plantlets were
successfully transferred to soil after hardening,
with a high rate of survival. The plants were
comparable to natural population in growth and
vigour [Prajapati A Hiren, Mehta R Saurabh and
Subramanian RB, In
vitro regeneration in
C u r c u l i g o
orchioides Gaertn.-
An endangered
medicinal herb,
Phytomorphology,
2004, 54(1 & 2),
85-95]. |
| Page(s): | 297-298 |
| ISSN: | 0975-1092 (Online); 0972-592X (Print) |
| Source: | NPR Vol.4(4) [July-August 2005]
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