Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/8065
Title: <b>Tissue Culture</b>
Issue Date: Feb-2005
Publisher: CSIR
Abstract: <b><i><span style="font-family: Times-BoldItalic; color: rgb(35, 31, 32);">Luffa </span></i></b><span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">sponge, derived from <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">dried fruits of <b><i><span style="font-family: Times-BoldItalic; color: rgb(35, 31, 32);">Luffa aegyptiaca </span></i></b><b><span style="font-family: Garamond-BoldCondensed; color: rgb(35, 31, 32);">Mill.</span></b> <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">(Sponge gourd, Hindi – <i><span style="font-family: Times-Italic; color: rgb(35, 31, 32);">Ghiya tori</span></i><span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">), <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">could be a suitable matrix for tissue <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">culture in aseptic liquid culture media. To <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">test this, the response of aroid <b><i><span style="font-family: Times-BoldItalic; color: rgb(35, 31, 32);">Philodendron </span></i></b><span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">‘Xanadu’ Hort., an <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">exquisite indoor-ornamental with its <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">beautiful leaves and tangled aerial roots, <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">was studied <i><span style="font-family: Times-Italic; color: rgb(35, 31, 32);">in vitro </span></i><span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">by Gangopadhyay and <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">others. A study on <i><span style="font-family: Times-Italic; color: rgb(35, 31, 32);">in vitro </span></i><span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">root <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">development in conventional agar-gelled <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">media in comparison to liquid media with <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">two alternative matrices like <b><i><span style="font-family: Times-BoldItalic; color: rgb(35, 31, 32);">Luffa</span></i></b> <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">sponge and coir indicated the suitability <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">of <b><i><span style="font-family: Times-BoldItalic; color: rgb(35, 31, 32);">Luffa </span></i></b><span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">sponge over the others. The <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">clonal uniformity of the micropropagated <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">plant was thus substantiated through <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">uniformity in RAPD (Random Amplified <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">Polymorphic DNA) profiles. For <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">commercial mass propagation where costeffectiveness <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">is a major check point, the <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">technique of RAPD being relatively <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">cheaper than other molecular techniques, <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">will definitely encourage scientists to adopt <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">it worldwide for quality assurance of the <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">tissue culture clones. The plants cultured <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">in liquid medium with <b><i><span style="font-family: Times-BoldItalic; color: rgb(35, 31, 32);">Luffa </span></i></b><span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">sponge as <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">the matrix showed high rate of survival <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">after transplantation, thus economizing the <span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">whole endeavour [Gangopadhyay <i><span style="font-family: Times-Italic; color: rgb(35, 31, 32);">et al,</span></i> <i><span style="font-family: Times-Italic; color: rgb(35, 31, 32);">Curr Sci</span></i><span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">, 2004, <b><span style="font-family: Garamond-BoldCondensed; color: rgb(35, 31, 32);">86</span></b><span style="font-family: Garamond-BookCondensed; color: rgb(35, 31, 32);">(2), 315-319]. </span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>
Description: 65-66
URI: http://hdl.handle.net/123456789/8065
ISSN: 0975-1092 (Online); 0972-592X (Print)
Appears in Collections:NPR Vol.4(1) [January-February 2005]

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