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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)] >
NPR Vol.4 [2005] >
NPR Vol.4(1) [January-February 2005] >
| Title: | Tissue Culture |
| Issue Date: | Feb-2005 |
| Publisher: | CSIR |
| Abstract: | Luffa sponge, derived from
dried fruits of Luffa aegyptiaca Mill.
(Sponge gourd, Hindi – Ghiya tori),
could be a suitable matrix for tissue
culture in aseptic liquid culture media. To
test this, the response of aroid
Philodendron ‘Xanadu’ Hort., an
exquisite indoor-ornamental with its
beautiful leaves and tangled aerial roots,
was studied in vitro by Gangopadhyay and
others. A study on in vitro root
development in conventional agar-gelled
media in comparison to liquid media with
two alternative matrices like Luffa
sponge and coir indicated the suitability
of Luffa sponge over the others. The
clonal uniformity of the micropropagated
plant was thus substantiated through
uniformity in RAPD (Random Amplified
Polymorphic DNA) profiles. For
commercial mass propagation where
costeffectiveness
is a major check point, the
technique of RAPD being relatively
cheaper than other molecular techniques,
will definitely encourage scientists to
adopt
it worldwide for quality assurance of the
tissue culture clones. The plants cultured
in liquid medium with Luffa sponge as
the matrix showed high rate of survival
after transplantation, thus economizing the
whole endeavour [Gangopadhyay et al,
Curr Sci, 2004, 86(2), 315-319].
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| Page(s): | 65-66 |
| ISSN: | 0975-1092 (Online); 0972-592X (Print) |
| Source: | NPR Vol.4(1) [January-February 2005]
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