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Title: Tissue Culture
Issue Date: Feb-2005
Publisher: CSIR
Abstract: Luffa sponge, derived from dried fruits of Luffa aegyptiaca Mill. (Sponge gourd, Hindi – Ghiya tori), could be a suitable matrix for tissue culture in aseptic liquid culture media. To test this, the response of aroid Philodendron ‘Xanadu’ Hort., an exquisite indoor-ornamental with its beautiful leaves and tangled aerial roots, was studied in vitro by Gangopadhyay and others. A study on in vitro root development in conventional agar-gelled media in comparison to liquid media with two alternative matrices like Luffa sponge and coir indicated the suitability of Luffa sponge over the others. The clonal uniformity of the micropropagated plant was thus substantiated through uniformity in RAPD (Random Amplified Polymorphic DNA) profiles. For commercial mass propagation where costeffectiveness is a major check point, the technique of RAPD being relatively cheaper than other molecular techniques, will definitely encourage scientists to adopt it worldwide for quality assurance of the tissue culture clones. The plants cultured in liquid medium with Luffa sponge as the matrix showed high rate of survival after transplantation, thus economizing the whole endeavour [Gangopadhyay et al, Curr Sci, 2004, 86(2), 315-319].
Page(s): 65-66
ISSN: 0975-1092 (Online); 0972-592X (Print)
Appears in Collections:NPR Vol.4(1) [January-February 2005]

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