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Title: Establishment and maintenance of callus of Stevia rebaudiana Bertoni under aseptic environment
Authors: Das, Kuntal
Dang, Raman
Rajasekharan, P E
Keywords: Stevia rebaudiana;Stevioside;Phytohormones;Growth regulators;Tissue culture;Callus;Aseptic environment
Issue Date: Oct-2006
Publisher: CSIR
IPC Code: Int. cl.8 A01H 4/00, A23L 2/60, A61K 36/00, A61K 36/28
Abstract: The callus culture of leaves of Stevia rebaudiana Bertoni (Family: Asteraceae) was initiated and maintained on different strengths of Murashiage and Skoog (MS) medium with phytohormones. The study showed that Kinetin in combination with Naphthalene Acetic Acid (NAA) and 2, 4-D exhibits better results for callus initiation whereas the combined application of Benzyl adenine or 6-benzyl amino purine (BAP) and NAA showed most satisfactory performance in maintaining callus when half strength of MS medium was used.
Page(s): 373-376
ISSN: 0975-1092 (Online); 0972-592X (Print)
Appears in Collections:NPR Vol.5(5) [September-October 2006]

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