NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Natural Products and Resources (IJNPR)
[Formerly Natural Product Radiance (NPR)]
NPR Vol.5 [2006] >
NPR Vol.5(1) [January-February 2006] >

Title: Benzyl amino purine and adenine sulphate induced rapid multiple shoot and root induction from nodal explants of Brahmi [Bacopa monnieri (Linn.)Penn.]
Authors: Ramesh, M
Saravanakumar, R M
Pandian, S Karutha
Keywords: Adenine sulphate
Bacopa monnieri
Callus induction
Multiple shoots
Benzyl Amino Purine (BAP)
Indole-3-Acetic Acid (IAA)
Naphthalene Acetic Acid (NAA)
Nodal explants
Issue Date: Feb-2006
Publisher: CSIR
IPC CodeInt. cl.7 A01H 4/00, A01H 5/04, A01H 5/06, A61K 35/78
Abstract: In vitro multiple shoot and root induction of Brahmi [Bacopa monnieri (Linn.) Penn.] was achieved from nodal explants. Shoot segments obtained from field grown plants were surface sterilized and cultured on Murashige and Skoog’s (MS) medium without any hormones to get regular supply of node explants. In order to find out the suitable conditions for propagation from nodal explants, MS medium with different combinations of auxins and cytokinins were tested. All the treatments were found effective in inducing shoots except those with auxin/cytokinin alone. The percent response of 92-100 was observed in 90% of treatments. The effect of special cytokinin like adenine sulphate on multiple shoot formation from the nodal explants of B. monnieri was tested on MS medium fortified with different hormonal concentrations of auxin and cytokinin. High percentage of multiple shoot formation (96.3) and maximum number of shoots (16.33) was observed in the combinational treatment of IAA (0.2 mg/l) and BAP (1.5 mg/l). Addition of adenine sulphate at 60mg/l along with IAA (0.2mg /l) and BAP (1.5mg/l) was found most effective in inducing highest number of shoots (18). All the other tested combinations have little effect on increasing the number of shoots. In vitro propagated plants were successfully transplanted to plastic pots containing sterile soil with 85% survival rate and later established under ex vitro condition.
Page(s): 44-51
ISSN: 0975-1092 (Online); 0972-592X (Print)
Source:NPR Vol.5(1) [January-February 2006]

Files in This Item:

File Description SizeFormat
NPR 5(1) 44-51.pdf1.85 MBAdobe PDFView/Open
 Current Page Visits: 897 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 171579 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: