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NPR Vol.5(4) [July-August 2006] >


Title: Establishment and Economic evaluation of micropropagated Jeewanti (Leptadenia reticulata Wight & Arn.) plants in field
Authors: Shekhawat, N S
Kackar, A
Rathore, M S
Singh, M
Dagla, H R
Arya, Vinod
Keywords: Leptadenia reticulata
Jeewanti
Micropropagation
Medicinal plant
Nodal segments
Ex vitro
Field trials
Economic evaluation
Issue Date: Aug-2006
Publisher: CSIR
IPC CodeInt. cl.7— A01H 5/04, A61K 35/78
Abstract:  Leptadenia reticulata Wight & Arn. is an important medicinal plant. There is heavy demand of the plant and its biomass which can not be fulfilled by the natural/ present practices of propagation. Natural propagation of this plant is poor and the plant is threatened in nature. Thus, there is a need for applying biotechnological methods for large scale propagation. Micropropagation studies were carried out by the authors on explants. Multiple shoots were differentiated on Murashige and Skoog’s (MS) medium containing 5.0 mg/litre 6-benzylaminopurine (BAP). The shoots further multiplied on MS medium + 1.5 mg/litre BAP and 0.5 mg/litre kinetin (KN) and the cloned shoots, treated with 200 mg/litre of Indole-3-butyric acid (IBA), rooted ex vitro. The plantlets were transferred to bottles containing soilrite moistened with half-strength MS macro salts. The hardened plants were transferred to polybags and kept in shade house for acclimatization. Subsequently field trials were carried out at villages Manai (Jodhpur) and Kachholi (Sirohi) of Rajasthan. In the present paper establishment of micropropagated plants, cultivation, growth and net profit from biomass produced has been reported for environmental conditions of Rajasthan. Total dry biomass harvested was 2800 kg/acre/ for first year and 3000 kg/acre/for second year.
Page(s): 311-314
ISSN: 0975-1092 (Online); 0972-592X (Print)
Source:NPR Vol.5(4) [July-August 2006]

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