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Journal of Scientific and Industrial Research (JSIR) >
JSIR Vol.67 [2008] >
JSIR Vol.67(04) [April 2008] >
| Title: | Fabrication of Pt based amperometric cholesterol biosensor using cellulose acetate membrane |
| Authors: | Vikas
Pundir, C S |
| Keywords: | Biosensor
Cellulose acetate
Cholesterol esterase
Cholesterol oxidase
Horseradish peroxidase
Pt electrode |
| Issue Date: | Apr-2008 |
| Publisher: | CSIR |
| Abstract: | Cholesterol esterase, cholesterol oxidase and horseradish peroxidase (HRP) have been co-immobilized onto cellulose
acetate membrane, which is mounted over biosensor, Pt electrode. Biosensor showes excellent performance with a linear
response between 2.5 to 50 mg dl⁻¹ of cholesterol acetate [correlation coefficient (r) = 0.9912]. Mean analytical recovery of
added cholesteryl acetate (100 and 200 mg dl⁻¹) in serum (n=6.0) has been found to be 99.86±0.78 and 97.79±0.73. Cholesterol
content of sera within batch and between batch coefficient of variation (CV) are <3% and < 5 %, respectively. Km for cholesterol
acetate and Vmax are 1083 mg/dl⁻¹ and 0.5 mA/sec. Optimum pH of biosensor is 7.0 and temperature 45°C. Enzyme electrode
lost 50% of its activity during its regular use for 150 times over a period of 25 days at 4°C. The response time is 35 second. Uric
acid and ascorbic acid shows slight interference. |
| Page(s): | 299-306 |
| ISSN: | 0022-4456 |
| Source: | JSIR Vol.67(04) [April 2008]
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