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Indian Journal of Chemistry -Section B (IJC-B) >
IJC-B Vol.49B [2010] >
IJC-B Vol.49B(04) [April 2010] >
| Title: | Purification of lignin peroxidase from Hexagona tenuis MTCC-1119 and its kinetic properties in aqueous medium containing miscible organic solvents |
| Authors: | Yadav, Meera
Singh, Sunil Kumar
Yadav, Kamlesh Singh
Yadav, Kapil Deo Singh |
| Keywords: | Lignin peroxidase
veratryl alcohol
Hexagona tenuis, n-propanol |
| Issue Date: | Apr-2010 |
| Publisher: | CSIR |
| Abstract: |
Lignin peroxidase from
culture filtrate of a new lignolytic fungal strain Hexagona tenuis
MTCC-1119 has been purified to homogeneity using a simple procedure. The
molecular wt. of the enzyme has been found to be 48 kDa using SDS-PAGE
analysis. The enzymatic characteristics like Km, pH and temperature optima of the enzyme using veratryl alcohol, n-propanol and H2O2
as substrate has been determined. The Km values have been found to be 70μM,
80μM and 530μM for veratryl alcohol, H2O2 and n-propanol respectively. The pH and temperature optima were 3 and 30°C respectively. The enzyme
retains 50% of its activity in 10% of the water miscible organic solvents
acetone, dioxane, diethyl ether, acetonitrile and dimethyl formamide in aqueous
medium. The inhibition of the enzyme by acetone has been found to be reversible
and uncompetitive with inhibition constant KI values 2.72 mM, 2.96 mM and 0.34 mM using veratryl
alcohol, hydrogen peroxide and n-propanol
as the substrates respectively.
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| Page(s): | 489-494 |
| Source: | IJC-B Vol.49B(04) [April 2010]
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