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Title: Purification of lignin peroxidase from Hexagona tenuis MTCC-1119 and its kinetic properties in aqueous medium containing miscible organic solvents
Authors: Yadav, Meera
Singh, Sunil Kumar
Yadav, Kamlesh Singh
Yadav, Kapil Deo Singh
Keywords: Lignin peroxidase;veratryl alcohol;Hexagona tenuis, n-propanol
Issue Date: Apr-2010
Publisher: CSIR
Abstract:  Lignin peroxidase from culture filtrate of a new lignolytic fungal strain Hexagona tenuis MTCC-1119 has been purified to homogeneity using a simple procedure. The molecular wt. of the enzyme has been found to be 48 kDa using SDS-PAGE analysis. The enzymatic characteristics like Km, pH and temperature optima of the enzyme using veratryl alcohol, n-propanol and H2O2 as substrate has been determined. The Km values have been found to be 70μM, 80μM and 530μM for veratryl alcohol, H2O2 and n-propanol respectively. The pH and temperature optima were 3 and 30°C respectively. The enzyme retains 50% of its activity in 10% of the water miscible organic solvents acetone, dioxane, diethyl ether, acetonitrile and dimethyl formamide in aqueous medium. The inhibition of the enzyme by acetone has been found to be reversible and uncompetitive with inhibition constant KI values 2.72 mM, 2.96 mM and 0.34 mM using veratryl alcohol, hydrogen peroxide and n-propanol as the substrates respectively.
Page(s): 489-494
Appears in Collections:IJC-B Vol.49B(04) [April 2010]

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