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Title: Effect of N6-Benzyl amino purine and Naphthalene acetic acid on camptothecin production through in vitro propagation of Ophiorrhiza rugosa Wall. var. decumbens (Gardn. ex Thw.) Deb & Mondal
Authors: Vineesh, V R
Jelly, C L
Fijesh, P V
Jaimsha, V K
Padikkala, J
Keywords: Ophiorrhiza rugosa var. decumbens;Camptothecin;10-Hydroxycamptothecin;Multiple shoot cultures;Root cultures;N6-Benzyl Amino Purine;Naphthalene acetic acid
Issue Date: Oct-2007
Publisher: CSIR
IPC Code: Int. cl.8 A01H 4/00, A01H 5/04, A01H 5/06
Abstract: In vitro production of camptothecin (CPT) was achieved by the establishment of multiple shoot and root cultures of Ophiorrhiza rugosa Wall. var. decumbens (Gardn. ex Thw.) Deb & Mondal. The maximum multiple shoots were obtained in Murashige and Skoog (MS) medium supplemented with 5mg/l N6-benzyl amino purine (BA) along with 0.5mg/l naphthalene acetic acid (NAA). Optimal root generation was observed in Murashige and Skoog medium with 2mg/l NAA and 0.05mg/l BA. However, CPT content found to be elevated in shoot and root cultures where growth hormones imposed growth inhibition. The maximum amount of CPT in multiple shoots was 0.039% while shoots of plant grown in the field showed 0.002 per cent. In root cultures, CPT content was 0.065% whereas roots of intact plant showed only 0.024 per cent.
Page(s): 405-409
ISSN: 0975-1092 (Online); 0972-592X (Print)
Appears in Collections:NPR Vol.6(5) [September-October 2007]

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