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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Natural Products and Resources (IJNPR)
[Formerly Natural Product Radiance (NPR)] >
NPR Vol.6 [2007] >
NPR Vol.6(5) [September-October 2007] >
| Title: | Effect of N6-Benzyl amino purine and Naphthalene acetic acid on camptothecin production through in vitro propagation of Ophiorrhiza rugosa Wall. var. decumbens (Gardn. ex Thw.) Deb & Mondal |
| Authors: | Vineesh, V R
Jelly, C L
Fijesh, P V
Jaimsha, V K
Padikkala, J |
| Keywords: | Ophiorrhiza rugosa var. decumbens
Camptothecin
10-Hydroxycamptothecin
Multiple shoot cultures
Root cultures
N6-Benzyl Amino Purine
Naphthalene acetic acid |
| Issue Date: | Oct-2007 |
| Publisher: | CSIR |
| IPC Code: | Int. cl.8 — A01H 4/00, A01H 5/04, A01H 5/06 |
| Abstract: | In
vitro production of camptothecin (CPT) was achieved by the establishment of multiple shoot and root cultures of Ophiorrhiza rugosa
Wall. var. decumbens (Gardn. ex Thw.) Deb & Mondal. The maximum multiple
shoots were obtained in Murashige and Skoog (MS) medium supplemented with 5mg/l
N6-benzyl amino purine (BA) along
with 0.5mg/l naphthalene acetic acid (NAA). Optimal root generation
was observed in Murashige and Skoog medium with 2mg/l NAA and 0.05mg/l BA. However, CPT
content found to be elevated in shoot and root cultures where growth hormones
imposed growth inhibition. The maximum amount of CPT in multiple shoots was
0.039% while shoots of plant grown in the field showed 0.002 per cent. In root
cultures, CPT content was 0.065% whereas roots of intact plant showed only 0.024
per cent. |
| Page(s): | 405-409 |
| ISSN: | 0975-1092 (Online); 0972-592X (Print) |
| Source: | NPR Vol.6(5) [September-October 2007]
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