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Title: <b>Effect of <i>N<sup>6</sup></i>-Benzyl amino purine and Naphthalene acetic acid on camptothecin production through <i>in vitro</i> propagation of </b> <b><i>Ophiorrhiza</i></b><b><i> rugosa </i>Wall.</b><b> var.<i> decumbens</i> (Gardn. ex Thw.) Deb & Mondal </b> <b> </b>
Authors: Vineesh, V R
Jelly, C L
Fijesh, P V
Jaimsha, V K
Padikkala, J
Keywords: <i>Ophiorrhiza</i><i> rugosa</i> var. <i>decumbens</i>
Multiple shoot cultures
Root cultures
N<sup>6</sup>-Benzyl Amino Purine
Naphthalene acetic acid
Issue Date: Oct-2007
Publisher: CSIR
Series/Report no.: <b>Int. cl.<sup>8</sup> </b><b>—</b> A01H 4/00, A01H 5/04, A01H 5/06
Abstract: <i>In vitro</i> production of camptothecin (CPT) was achieved by the establishment of multiple shoot and root cultures of <i>Ophiorrhiza</i><i> rugosa </i>Wall. var. <i>decumbens</i><i> </i>(Gardn. ex Thw.) Deb & Mondal. The maximum multiple shoots were obtained in Murashige and Skoog (MS) medium supplemented with 5mg/l <i>N</i><sup>6</sup>-benzyl amino purine (BA) along with 0.5mg/l<sup> </sup>naphthalene acetic acid (NAA). Optimal root generation was observed in Murashige and Skoog medium with 2mg/l NAA and 0.05mg/l BA. However, CPT content found to be elevated in shoot and root cultures where growth hormones imposed growth inhibition. The maximum amount of CPT in multiple shoots was 0.039% while shoots of plant grown in the field showed 0.002 per cent. In root cultures, CPT content was 0.065% whereas roots of intact plant showed only 0.024 per cent.
Description: 405-409
ISSN: 0975-1092 (Online); 0972-592X (Print)
Appears in Collections:NPR Vol.6(5) [September-October 2007]

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