<b>Effect of <i>N⁶</i>-Benzyl amino purine and Naphthalene acetic acid on camptothecin production through <i>in vitro</i> propagation of </b> <b><i>Ophiorrhiza</i></b><b><i> rugosa </i>Wall.</b><b> var.<i> decumbens</i> (Gardn. ex Thw.) Deb & Mondal </b> <b> </b>

Abstract

<i>In vitro</i> production of camptothecin (CPT) was achieved by the establishment of multiple shoot and root cultures of <i>Ophiorrhiza</i><i> rugosa </i>Wall. var. <i>decumbens</i><i> </i>(Gardn. ex Thw.) Deb & Mondal. The maximum multiple shoots were obtained in Murashige and Skoog (MS) medium supplemented with 5mg/l <i>N</i>⁶-benzyl amino purine (BA) along with 0.5mg/lnaphthalene acetic acid (NAA). Optimal root generation was observed in Murashige and Skoog medium with 2mg/l NAA and 0.05mg/l BA. However, CPT content found to be elevated in shoot and root cultures where growth hormones imposed growth inhibition. The maximum amount of CPT in multiple shoots was 0.039% while shoots of plant grown in the field showed 0.002 per cent. In root cultures, CPT content was 0.065% whereas roots of intact plant showed only 0.024 per cent.