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Title: | Effect of N6-Benzyl amino purine and Naphthalene acetic acid on camptothecin production through in vitro propagation of Ophiorrhiza rugosa Wall. var. decumbens (Gardn. ex Thw.) Deb & Mondal |
Authors: | Vineesh, V R Jelly, C L Fijesh, P V Jaimsha, V K Padikkala, J |
Keywords: | Ophiorrhiza rugosa var. decumbens;Camptothecin;10-Hydroxycamptothecin;Multiple shoot cultures;Root cultures;N6-Benzyl Amino Purine;Naphthalene acetic acid |
Issue Date: | Oct-2007 |
Publisher: | CSIR |
IPC Code: | Int. cl.8 — A01H 4/00, A01H 5/04, A01H 5/06 |
Abstract: | In vitro production of camptothecin (CPT) was achieved by the establishment of multiple shoot and root cultures of Ophiorrhiza rugosa Wall. var. decumbens (Gardn. ex Thw.) Deb & Mondal. The maximum multiple shoots were obtained in Murashige and Skoog (MS) medium supplemented with 5mg/l N6-benzyl amino purine (BA) along with 0.5mg/l naphthalene acetic acid (NAA). Optimal root generation was observed in Murashige and Skoog medium with 2mg/l NAA and 0.05mg/l BA. However, CPT content found to be elevated in shoot and root cultures where growth hormones imposed growth inhibition. The maximum amount of CPT in multiple shoots was 0.039% while shoots of plant grown in the field showed 0.002 per cent. In root cultures, CPT content was 0.065% whereas roots of intact plant showed only 0.024 per cent. |
Page(s): | 405-409 |
URI: | http://hdl.handle.net/123456789/7894 |
ISSN: | 0975-1092 (Online); 0972-592X (Print) |
Appears in Collections: | NPR Vol.6(5) [September-October 2007] |
Files in This Item:
File | Description | Size | Format | |
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NPR 6(5) 405-409.pdf | 227.08 kB | Adobe PDF | View/Open |
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