NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Natural Products and Resources (IJNPR)
[Formerly Natural Product Radiance (NPR)]
 >
NPR Vol.6 [2007] >
NPR Vol.6(4) [July-August 2007] >


Title: Protection from oxidative damage using Emblica officinalis Gaertn. extracts in case of ochratoxin induced toxicity in normal human RBC
Authors: Verma, R J
Chakraborty, Devjani
Keywords: Indian gooseberry
Ochratoxin
Emblica officinalis
Amla
Human RBC
Haemolysis
Oxidative damage
Antioxidant
Issue Date: Aug-2007
Publisher: CSIR
IPC CodeInt. cl.8 — A61K 36/00, A61P 7/00, A61P 39/06
Abstract: Treatment of saline suspension of human RBC with crude ochratoxin, obtained from culture filtrate of Aspergillus ochraceus (ITCFF, IARI, NO-1456) for 4 hours at 37ºC, produced pronounced swelling and haemolysis. Haemolysis was more at higher concentration (4-5 µg/ml). The ambient suspension turned red due to release of haemoglobin from haemolysed corpuscles. The haemolysis was reduced significantly by the aqueous and alcoholic extract of Emblica officinalis Gaertn. (Amla) when administered along with the ochratoxin. The retarding effect was increased with the increase in concentration of aqueous extract from 5-70 µg/ml and alcoholic extract from 5-60 µg/ml. The aqueous and alcoholic extract of amla has powerful retarding effect on ochratoxin-induced haemolysis on RBC. This may be because of the presence of vitamin-C, tannins and flavonoids, which are believed to be potent antioxidants found in amla. RBC has got the simplest structure and can be used as a very good model to detect the direct effect of a toxin on the cell membrane. Destabilization of the cell membrane in RBC can leads to lysis of the cell and release of haemoglobin in the medium. Extent of haemolysis can help to reveal the extent of toxicity
Page(s): 310-314
ISSN: 0975-1092 (Online); 0972-592X (Print)
Source:NPR Vol.6(4) [July-August 2007]

Files in This Item:

File Description SizeFormat
NPR 6(4) 310-314.pdf177.16 kBAdobe PDFView/Open
 Current Page Visits: 540 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 623227 since 06-Feb-2009  Last updated on 13-Nov-2014Webmaster: nopr@niscair.res.in