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Title: Protection from oxidative damage using Emblica officinalis Gaertn. extracts in case of ochratoxin induced toxicity in normal human RBC
Authors: Verma, R J
Chakraborty, Devjani
Keywords: Indian gooseberry;Ochratoxin;Emblica officinalis;Amla;Human RBC;Haemolysis;Oxidative damage;Antioxidant
Issue Date: Aug-2007
Publisher: CSIR
IPC Code: Int. cl.8 — A61K 36/00, A61P 7/00, A61P 39/06
Abstract: Treatment of saline suspension of human RBC with crude ochratoxin, obtained from culture filtrate of Aspergillus ochraceus (ITCFF, IARI, NO-1456) for 4 hours at 37ºC, produced pronounced swelling and haemolysis. Haemolysis was more at higher concentration (4-5 µg/ml). The ambient suspension turned red due to release of haemoglobin from haemolysed corpuscles. The haemolysis was reduced significantly by the aqueous and alcoholic extract of Emblica officinalis Gaertn. (Amla) when administered along with the ochratoxin. The retarding effect was increased with the increase in concentration of aqueous extract from 5-70 µg/ml and alcoholic extract from 5-60 µg/ml. The aqueous and alcoholic extract of amla has powerful retarding effect on ochratoxin-induced haemolysis on RBC. This may be because of the presence of vitamin-C, tannins and flavonoids, which are believed to be potent antioxidants found in amla. RBC has got the simplest structure and can be used as a very good model to detect the direct effect of a toxin on the cell membrane. Destabilization of the cell membrane in RBC can leads to lysis of the cell and release of haemoglobin in the medium. Extent of haemolysis can help to reveal the extent of toxicity
Page(s): 310-314
ISSN: 0975-1092 (Online); 0972-592X (Print)
Appears in Collections:NPR Vol.6(4) [July-August 2007]

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