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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Natural Products and Resources (IJNPR)
[Formerly Natural Product Radiance (NPR)] >
NPR Vol.6 [2007] >
NPR Vol.6(4) [July-August 2007] >
| Title: | Protection from oxidative damage using Emblica officinalis Gaertn. extracts in case of ochratoxin induced toxicity in normal human RBC |
| Authors: | Verma, R J
Chakraborty, Devjani |
| Keywords: | Indian gooseberry
Ochratoxin
Emblica officinalis
Amla
Human RBC
Haemolysis
Oxidative damage
Antioxidant |
| Issue Date: | Aug-2007 |
| Publisher: | CSIR |
| IPC Code: | Int. cl.8 — A61K 36/00, A61P 7/00, A61P 39/06 |
| Abstract: | Treatment of saline suspension of human RBC with crude ochratoxin, obtained from
culture filtrate of Aspergillus
ochraceus (ITCFF, IARI, NO-1456) for 4 hours at 37ºC, produced pronounced
swelling and haemolysis. Haemolysis was more at higher concentration (4-5
µg/ml). The ambient suspension turned red due to release of haemoglobin from
haemolysed corpuscles. The haemolysis was reduced significantly by the aqueous
and alcoholic extract of Emblica
officinalis Gaertn. (Amla) when administered along with the
ochratoxin. The retarding effect was increased with the increase in
concentration of aqueous extract from 5-70 µg/ml and alcoholic extract from 5-60
µg/ml. The aqueous and alcoholic extract of amla has powerful retarding
effect on ochratoxin-induced haemolysis on RBC. This may be because of the
presence of vitamin-C, tannins and flavonoids, which are believed to be potent
antioxidants found in amla. RBC has got the simplest structure and can be
used as a very good model to detect the direct effect of a toxin on the cell
membrane. Destabilization of the cell membrane in RBC can leads to lysis of the
cell and release of haemoglobin in the medium. Extent of haemolysis can help to
reveal the extent of toxicity |
| Page(s): | 310-314 |
| ISSN: | 0975-1092 (Online); 0972-592X (Print) |
| Source: | NPR Vol.6(4) [July-August 2007]
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