Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/7877
Title: <b style="">Protection from oxidative damage using <i style="">Emblica officinalis</i> Gaertn. extracts in case of ochratoxin induced toxicity in normal human RBC</b>
Authors: Verma, R J
Chakraborty, Devjani
Keywords: Indian gooseberry
Ochratoxin
<i>Emblica officinalis</i>
Amla
Human RBC
Haemolysis
Oxidative damage
Antioxidant
Issue Date: Aug-2007
Publisher: CSIR
Series/Report no.: <b>Int. cl.<sup>8</sup></b> — A61K 36/00, A61P 7/00, A61P 39/06
Abstract: Treatment of saline suspension of human RBC with crude ochratoxin, obtained from culture filtrate of <i style="">Aspergillus ochraceus</i> (ITCFF, IARI, NO-1456) for 4 hours at 37ºC, produced pronounced swelling and haemolysis. Haemolysis was more at higher concentration (4-5 µg/ml). The ambient suspension turned red due to release of haemoglobin from haemolysed corpuscles. The haemolysis was reduced significantly by the aqueous and alcoholic extract of <b><i style="">Emblica officinalis</i> Gaertn.</b> (<i>Amla</i>) when administered along with the ochratoxin. The retarding effect was increased with the increase in concentration of aqueous extract from 5-70 µg/ml and alcoholic extract from 5-60 µg/ml. The aqueous and alcoholic extract of <i>amla</i> has powerful retarding effect on ochratoxin-induced haemolysis on RBC. This may be because of the presence of vitamin-C, tannins and flavonoids, which are believed to be potent antioxidants found in <i>amla</i>. RBC has got the simplest structure and can be used as a very good model to detect the direct effect of a toxin on the cell membrane. Destabilization of the cell membrane in RBC can leads to lysis of the cell and release of haemoglobin in the medium. Extent of haemolysis can help to reveal the extent of toxicity
Description: 310-314
URI: http://hdl.handle.net/123456789/7877
ISSN: 0975-1092 (Online); 0972-592X (Print)
Appears in Collections:NPR Vol.6(4) [July-August 2007]

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