|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Natural Products and Resources (IJNPR)
[Formerly Natural Product Radiance (NPR)] >
NPR Vol.6 [2007] >
NPR Vol.6(4) [July-August 2007] >
| Title: | Process optimization for the extraction of hyperforin and hypericin from St. John’s Wort |
| Authors: | Koul, V K
Koul, Suman |
| Keywords: | Hypericum perforatum
St. John’s Wort
Hyperforin
Hypericin
Extraction
Optimization |
| Issue Date: | Aug-2007 |
| Publisher: | CSIR |
| IPC Code: | Int. cl.8 — A61K 36/00, A61K 31/00, A61P 25/24 |
| Abstract: | St. John’s Wort (Hypericum
perforatum Linn.), native to Europe and South America, grows wild in
temperate and central parts of India. It has been used as a medicinal plant
since ancient times for ulceration of kidneys, jaundice, gout and rheumatism.
The antidepressant activity of St. John’s Wort extract has sparked great
interest in the chemistry and biochemistry of its constituents, hyperforin and
hypericin. The process described in this paper outlines the extraction of
hyperforin by solvent hexane and thereafter extraction of hypericin from marc by
commercial ethanol. The process parameters optimized for both the extractions
are: solvent/plant material ratio, temperature, number of extractions and time
for each extraction. Optimization was carried out by performing different sets
of experiments (on 15kg scale) and varying one parameter at a time for each set
of experiment. Depending on quality of plant material, under optimum conditions
(i) Hexane extraction yielded 4 to 5% residue containing up to 38% Hyperforin,
and (ii) Ethanol extraction yielded 20 to 22% residue containing up to 2.5%
hypericin. |
| Page(s): | 293-296 |
| ISSN: | 0975-1092 (Online); 0972-592X (Print) |
| Source: | NPR Vol.6(4) [July-August 2007]
|
|
