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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(2) [April 2010] >
| Title: | Development, micropropagation and characterization of colchiploid of Echinacea purpurea (L.) Moench |
| Authors: | Koul, Sushma
Sambyal, Manju
Kitchlu, S K
Bakshi, S K
Kaul, M K |
| Keywords: | Caffeic acid
Echinacea purpurea
micropropagation
polyploidization
tetraploid |
| Issue Date: | Apr-2010 |
| Publisher: | CSIR |
| Abstract: |
To enhance the genetic resource base of Echinacea purpurea (L.)
Moench, a tetraploid was developed by treating seeds with different
concentrations of colchicine. For further multiplication, in vitro micropropagation through adventitious shoot induction and proliferation was achieved. Shoot buds were induced directly on adaxial surface of
mature leaf tissues growing on MS medium supplemented with equimolar
concentration of BAP and IBA. The optimum initial shoot regeneration frequency
(63%) with average 2.3 shoots per explant was achieved after 30 d of culture
initiation. Maximum shoot organogenesis,
with 8-10 shoots per culture flask, was obtained on the same medium after 10th
subculture. Proliferating callus with intermixed shoot buds was derived from
leaf tissue explants placed on MS medium supplemented with 1 mg L-1 each of BAP and IAA. The regenerated shoots were
rooted on MS medium devoid of any growth regulator. Rooted plantlets were
hardened under greenhouse conditions at 20-22°C with 85-90% RH. About 95% plantlets survived
acclimatization, producing phenotypically normal plants in the greenhouse.
After 4 wks, rooted plants were successfully transferred to soil in
experimental field station at Jammu.
Comparable field trials were laid with parental diploids. Tetraploids were
dwarf, showed low seed set but were superior because of higher caffeic acid
content. The
results of this study have established a procedure for development and
propagation of tetraploid of E. purpurea having higher caffeic acid
content. |
| Page(s): | 221-224 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(2) [April 2010]
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