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Title: An efficient protocol for <i style="">in vitro</i> regeneration of <i>Podophyllum hexandrum</i>, a critically endangered medicinal plant
Authors: Chakraborty, Amrita
Bhattacharya, Dipto
Ghanta, Srijani
Chattopadhyay, Sharmila
Keywords: <i>Podophyllum hexandrum</i>
rhizome explants
endangered medicinal plant
<i>in vitro</i> propagation
Issue Date: Apr-2010
Publisher: CSIR
Abstract: Rhizome explants were used to develop a protocol for <i style="">in vitro</i> plantlet regeneration of <i>Podophyllum hexandrum</i>, a critically endangered medicinal plant, through direct organogenesis. Highest rate of multiple shoot formation was noted in MS medium supplemented with 11.42 μ<i style="">M</i> IAA within three months. A synergistic effect of 2.68 μ<i style="">M</i> NAA and 11.1 μ<i style="">M</i> BAP was second best. The ½ MS liquid medium with 100 μ<i style="">M </i>IBA was most suitable for rooting of shoots. Leaf explants resulted in callus formation only. Leaf regenerated calli showed the presence of podophyllotoxin in HPLC analysis.
Description: 217-220
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.09(2) [April 2010]

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