NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(2) [April 2010] >


Title: An efficient protocol for in vitro regeneration of Podophyllum hexandrum, a critically endangered medicinal plant
Authors: Chakraborty, Amrita
Bhattacharya, Dipto
Ghanta, Srijani
Chattopadhyay, Sharmila
Keywords: Podophyllum hexandrum
rhizome explants
endangered medicinal plant
conservation
in vitro propagation
podophyllotoxin
Issue Date: Apr-2010
Publisher: CSIR
Abstract: Rhizome explants were used to develop a protocol for in vitro plantlet regeneration of Podophyllum hexandrum, a critically endangered medicinal plant, through direct organogenesis. Highest rate of multiple shoot formation was noted in MS medium supplemented with 11.42 μM IAA within three months. A synergistic effect of 2.68 μM NAA and 11.1 μM BAP was second best. The ½ MS liquid medium with 100 μM IBA was most suitable for rooting of shoots. Leaf explants resulted in callus formation only. Leaf regenerated calli showed the presence of podophyllotoxin in HPLC analysis.
Page(s): 217-220
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.09(2) [April 2010]

Files in This Item:

File Description SizeFormat
IJBT 9(2) 217-220.pdf270.9 kBAdobe PDFView/Open
 Current Page Visits: 902 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 554601 since 06-Feb-2009  Last updated on 15-Jul-2014Webmaster: nopr@niscair.res.in