Please use this identifier to cite or link to this item:
|Title:||An efficient protocol for <i style="">in vitro</i> regeneration of <i>Podophyllum hexandrum</i>, a critically endangered medicinal plant|
endangered medicinal plant
<i>in vitro</i> propagation
|Abstract:||Rhizome explants were used to develop a protocol for <i style="">in vitro</i> plantlet regeneration of <i>Podophyllum hexandrum</i>, a critically endangered medicinal plant, through direct organogenesis. Highest rate of multiple shoot formation was noted in MS medium supplemented with 11.42 μ<i style="">M</i> IAA within three months. A synergistic effect of 2.68 μ<i style="">M</i> NAA and 11.1 μ<i style="">M</i> BAP was second best. The ½ MS liquid medium with 100 μ<i style="">M </i>IBA was most suitable for rooting of shoots. Leaf explants resulted in callus formation only. Leaf regenerated calli showed the presence of podophyllotoxin in HPLC analysis.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.09(2) [April 2010]|
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.