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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(2) [April 2010] >
| Title: | Phenotypic characters of various off types identified in laboratory, primary and secondary hardening in tissue cultured banana var. Grand naine |
| Authors: | Vasane, Shailesh
Patil, Anil
Kothari, R M |
| Keywords: | Banana
Grand naine
off types
somoclonal variation
morphological profiles
tissue culture |
| Issue Date: | Apr-2010 |
| Publisher: | CSIR |
| Abstract: | An
integrated study was undertaken on micropropagation of Grand nain variety of
banana (Musa paradisiaca) from elite plants to identify
and segregate (i) 3 types of off plants at the end of growth room phase
development, (ii) 5 types of off plants at the end of primary hardening phase
and (iii) totally 9 types (which included common 5 types observed at the end of
primary hardening) at the end of secondary hardening phase. The morphological
profiles of normal and off type plants were studied as a function of the above
mentioned growth phases. The 9 off types identified after secondary hardening
were finally subjected to field trials along with normal plants to serve as
control using an optimized protocol of fertilizer application and irrigation.
Their outcome indicated that off types, regardless of the type, could not
sustain either transplantation shock or survived for not more that 3-6 months,
giving poor growth/ no fruiting/ poor yield in quality and quantity. The
composite data indicated that these off types were somoclonal variants and were
not the result of epigenetic factor(s), as ruled out from studies of factors
likely to affect their growth. It is, therefore, prudent to segregate them
rigorously at each developmental phase so that delivery of only healthy normal
plants to farmers was assured, limiting to minimum, if any, inadvertent
contamination of off type plants due to limitation in their identification. |
| Page(s): | 178-186 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(2) [April 2010]
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