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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(2) [April 2010] >
| Title: | Degradation of flatulence-causing oligosaccharides in soymilk by α-galactosidase—A novel thermotolerant from Penicillium purpurogenum |
| Authors: | Ramalingam
Rudra
Saraswathy, N
Sadasivam, S |
| Keywords: | Characterization
α-galactosidase
purification
raffinose
soymilk
stachyose |
| Issue Date: | Apr-2010 |
| Publisher: | CSIR |
| Abstract: | α-Galactosidase from Penicillium purpurogenum was induced to a greater extent in the
culture medium by arabinose, galactose, lactose and guar galactomannan. The
enzyme was purified by acetone precipitation and DEAE-Sephacel column
chromatography. The purified enzyme showed a single band in both native-PAGE
and SDS-PAGE. The molecular mass of enzyme was found to be between 107 and 110
kDa. The enzyme exhibited the optimum pH and temperature at 5.0 and
55ºC, respectively. α-Galactosidase was strongly inhibited by Ag+
and Hg2+, while Fe2+ enhanced the activity. PMSF did not
inhibit α-galactosidase activity, whereas N-bromosuccinimide completely
inhibited the enzyme activity. Further, galactose and melibiose moderately
inhibited the enzyme activity. The Km values for PNPG, melibiose,
raffinose and stachyose were found to be 0.20, 5.26, 10 and 9.1, respectively.
α-Galactosidase completely hydrolyzed flatulence-causing raffinose and
stachyose of soymilk in 1.45 h. |
| Page(s): | 160-165 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(2) [April 2010]
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