NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(2) [April 2010] >

Title: Degradation of flatulence-causing oligosaccharides in soymilk by α-galactosidase—A novel thermotolerant from Penicillium purpurogenum
Authors: Ramalingam
Saraswathy, N
Sadasivam, S
Keywords: Characterization
Issue Date: Apr-2010
Publisher: CSIR
Abstract: α-Galactosidase from Penicillium purpurogenum was induced to a greater extent in the culture medium by arabinose, galactose, lactose and guar galactomannan. The enzyme was purified by acetone precipitation and DEAE-Sephacel column chromatography. The purified enzyme showed a single band in both native-PAGE and SDS-PAGE. The molecular mass of enzyme was found to be between 107 and 110 kDa. The enzyme exhibited the optimum pH and temperature at 5.0 and 55ºC, respectively. α-Galactosidase was strongly inhibited by Ag+ and Hg2+, while Fe2+ enhanced the activity. PMSF did not inhibit α-galactosidase activity, whereas N-bromosuccinimide completely inhibited the enzyme activity. Further, galactose and melibiose moderately inhibited the enzyme activity. The Km values for PNPG, melibiose, raffinose and stachyose were found to be 0.20, 5.26, 10 and 9.1, respectively. α-Galactosidase completely hydrolyzed flatulence-causing raffinose and stachyose of soymilk in 1.45 h.
Page(s): 160-165
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.09(2) [April 2010]

Files in This Item:

File Description SizeFormat
IJBT 9(2) 160-165.pdf168.54 kBAdobe PDFView/Open
 Current Page Visits: 1295 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 170844 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: