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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(2) [April 2010] >
| Title: | Isolation, cloning and molecular characterization of polygalacturonase I (pgaI) gene from Aspergillus niger isolate from mango |
| Authors: | Mukadam, D S
Chavan, A M
Taware, A S
Taware, S D |
| Keywords: | Aspergillus
cloning
PCR
pga1 gene
polygalacturonase |
| Issue Date: | Apr-2010 |
| Publisher: | CSIR |
| Abstract: |
Isolation, cloning and molecular
characterization of polygalacturonase (pga1)
gene from the mango isolate of Aspergillus
niger
has been reported. The full length amplicon consisted of 1101 bp. The
entire cDNA gene with the predicted protein of 367 amino acids had an estimated
mol wt of 38.28 kDa with pI 4.40. When the nucleic acid sequence was compared
with other Aspergillus spp., pga1 sequence showed the highest
sequence similarity with A. niger
and
A. fumigatus. Comparison of the amino
acid sequences revealed the presence of high degree of homology among the
polygalacturonases (PGs) from different fungi. Bioinformatics analysis suggests
that nucleic acid sequence of the isolated pgaI
gene shares 98% homology with the pgaI
gene of A. niger.
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| Page(s): | 153-159 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(2) [April 2010]
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