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IJBT Vol.09(2) [April 2010] >


Title:  Isolation, cloning and molecular characterization of polygalacturonase I (pgaI) gene from Aspergillus niger isolate from mango
Authors: Mukadam, D S
Chavan, A M
Taware, A S
Taware, S D
Keywords: Aspergillus
cloning
PCR
pga1 gene
polygalacturonase
Issue Date: Apr-2010
Publisher: CSIR
Abstract:  Isolation, cloning and molecular characterization of polygalacturonase (pga1) gene from the mango isolate of Aspergillus niger has been reported. The full length amplicon consisted of 1101 bp. The entire cDNA gene with the predicted protein of 367 amino acids had an estimated mol wt of 38.28 kDa with pI 4.40. When the nucleic acid sequence was compared with other Aspergillus spp., pga1 sequence showed the highest sequence similarity with A. niger and A. fumigatus. Comparison of the amino acid sequences revealed the presence of high degree of homology among the polygalacturonases (PGs) from different fungi. Bioinformatics analysis suggests that nucleic acid sequence of the isolated pgaI gene shares 98% homology with the pgaI gene of A. niger.
Page(s): 153-159
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.09(2) [April 2010]

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