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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(2) [April 2010] >
| Title: | Genotyping of a mapping population derived from salt susceptible and salt tolerant varieties (IR 28  Pokkali) of rice |
| Authors: | Rajani, R
Das, Sonali
Choudhury, P Ray
Mandal, Asit B |
| Keywords: | Abiotic stress
diversity, genotyping
RAPD
rice
salt tolerance |
| Issue Date: | Apr-2010 |
| Publisher: | CSIR |
| Abstract: |
Rice is the most important staple food
crop in India
having wide adaptability across diverse agro-ecosystems. A set of recombinant population was developed by hybridizing IR 28 with
Pokkali to tag salt tolerant gene(s). Pokkali is a tall, traditional,
photosensitive, profuse tillering, lodging susceptible, extremely long duration,
low yielding cultivar. It is generally grown in the coastal area of Kerala
owing to high degree of tolerance towards salinity. It is being used as
international check for salinity tolerance screening trials. This cultivar
possesses long, broad, dark and droopy leaves. In contrast, IR 28 is a high
yielding, dwarf, medium grained, short duration variety, having extreme
sensitivity to salinity. This indica/indica
hydridization was designed to combine high yielding ability of IR 28 with high
salinity tolerance of Pokkali. Phenotyping of F3s and parents were
done under NaCl (12 dSm-1) stressed condition. Genotyping, using
random primers, showed ample polymorphism, which revealed that F3
segregants had high genetic variation and could be very useful as mapping
population to tag salt tolerant loci. Further, the study has also revealed that
RAPD is an efficient tool to display polymorphism and to tag the targeted
gene(s).
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| Page(s): | 147-152 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(2) [April 2010]
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