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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(2) [April 2010] >
| Title: | RAPD analysis in mungbean [Vigna radiata (L.) Wilczek]: I. Assessment of genetic diversity |
| Authors: | Saini, Madhu
Singh, Subhadra
Hussain, Z
Yadav, A |
| Keywords: | Cluster analysis
genetic diversity
green-gram
molecular analysis
principal coordinate analysis
RAPD |
| Issue Date: | Apr-2010 |
| Publisher: | CSIR |
| Abstract: | Genetic diversity was estimated among 39
genotypes of mungbean [Vigna radiata
(L.) Wilczek] using 30 RAPD primers. A total of 411 RAPD amplicons were
obtained, of which 382 (92.9%) were polymorphic. The per cent polymorphism
ranged from 42.85 to 100 per cent. A wide range (40.8 to 90.3%) of Jaccards
similarity coefficient was observed between the pairs of genotypes; the highest
being observed between MH-98-1 and Vs ML-839, and the lowest between UPM-98 and
Vs ML-131. The genotypes with a common ancestry and/or used repeatedly in
mungbean improvement programme exhibited higher genotypic similarity. The
extent of diversity among cultivars was also studied in relation to their
source, and set of genotypes with narrow genetic base developed from particular
place were identified. The genotypes formed 11 clusters, three main groups and
eight minor groups, based on unweighted pair group methods for arithmetic mean
(UPGMA). The cultivar ML-131 occupied a unique position and was most diverse
from rest of the genotypes. Principal coordinate analysis and normalized Mantel
statistics (r=0.92) supported cluster analysis. A concordance was observed between
the position of genotypes in the cluster and their pedigree information. |
| Page(s): | 137-146 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(2) [April 2010]
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