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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(2) [April 2010] >
| Title: | Identification of genetic variation at murine casein loci by RFLP |
| Authors: | George, Sisilamma
Ponnachan, P T C
Archibald, Alan L |
| Keywords: | Restriction enzymes
murine casein loci
RFLP
Mus musculus
M. spretus |
| Issue Date: | Apr-2010 |
| Publisher: | CSIR |
| Abstract: | Genetic variation in the casein loci of Mus musculus (8 different strains) and Mus spretus was examined as restriction
fragment length variants (RFLVs) using 5 restriction enzymes, BamHI, SftI, EcoRI, XbaI
and HindIII. No
variation was observed between the inbred strains of M. musculus. Between
M. musculus and M. spretus variations in fragment length were observed at different casein loci,
revealing restriction fragment length polymorphisms (RFLPs). All the 5 enzymes
generated polymorphic fragments in  -casein locus while  and  casein loci were polymorphic to
four enzymes. The enzymes, HindIII and XbaI, yielded monomorphic
fragments in and -casein loci, respectively. Three
enzymes generated polymorphic fragments in  -casein locus but only two yielded
polymorphic fragments at  -casein locus. Among the different casein loci, -casein locus was highly
polymorphic while -casein
locus exhibited least polymorphism. Thus, RFLVs suggest the occurrence of
similar alleles at all the five casein loci viz., , , , and of the inbred strains of
M. musculus and a bi-allelic existence was observed between M. musculus and M. spretus. |
| Page(s): | 131-136 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(2) [April 2010]
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