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Title: In vitro corm induction and genetic stability of regenerated plants in taro [Colocasia esculenta (L.) Schott]
Authors: Hussain, Z
Tyagi, R K
Keywords: conservation
genetic integrity
inter-simple sequence repeats
random amplified polymorphic DNA
tissue culture
Issue Date: Oct-2006
Publisher: CSIR
IPC CodeInt. Cl.8 A01H4/00, 5/04
Abstract: In vitro corm formation was achieved on Murashige and Skoog’s (MS) medium, containing 8-10% sucrose, 22 µM N6-benzyl aminopurine (BAP), 0.6 µM α-naphthaleneacetic acid (NAA) and 0.8% agar, in taro (IC 420791). The corm forming cultures could be conserved up to 15 months at 25+2oC, whereas shoot-forming cultures could last for only 6 months on MS medium, containing 3% sucrose + 2.2 µM BAP + 0.6 µM NAA + 0.8% agar. Plantlets with in vitro-formed corms showed 100% survival in the field, and developed normal uniform corm-producing plants. Uniformity of tissue culture regenerated plants with corm (Ro) was determined on the basis of 12 qualitative and 10 quantitative morphological traits related to leaf, petiole, corm and root. Additionally, two PCR-based markers—RAPD and ISSR were also applied to determine the genetic stability of Ro plants. A total of 13 RAPD primers (of 35 tested initially) and 6 ISSR primers gave 111 distinct bands in RAPD and 43 in ISSR, and exhibited uniform RAPD and ISSR banding patterns for Ro plants tested. Our results suggested that present protocol used for in vitro corm formation may cost-effectively be applied for conservation of taro germplasm along with maintaining the genetic stability and functionality of plants. Further possibility may also be explored to use this protocol for production of disease-free planting materials. This will facilitate the national and international exchange of taro germplasms.
Page(s): 535-542
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.05(4) [October 2006]

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