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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(4) [October 2006] >
| Title: | In vitro corm induction and genetic stability of regenerated plants in taro [Colocasia esculenta (L.) Schott] |
| Authors: | Hussain, Z
Tyagi, R K |
| Keywords: | conservation
genetic integrity
inter-simple sequence repeats
random amplified polymorphic DNA
taro
tissue culture |
| Issue Date: | Oct-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 A01H4/00, 5/04 |
| Abstract: | In
vitro corm formation was achieved on Murashige and
Skoog’s (MS) medium, containing 8-10% sucrose, 22 µM N6-benzyl aminopurine (BAP), 0.6 µM α-naphthaleneacetic acid (NAA) and 0.8% agar, in taro (IC
420791). The corm forming cultures could be conserved up to 15 months at 25+2oC,
whereas shoot-forming cultures could last for only 6 months on MS medium,
containing 3% sucrose + 2.2 µM BAP +
0.6 µM NAA + 0.8% agar. Plantlets
with in vitro-formed corms showed
100% survival in the field, and developed normal uniform corm-producing plants.
Uniformity of tissue culture regenerated plants with corm (Ro) was determined
on the basis of 12 qualitative and 10 quantitative morphological traits related
to leaf, petiole, corm and root. Additionally, two PCR-based markers—RAPD and
ISSR were also applied to determine the genetic stability of Ro plants. A total
of 13 RAPD primers (of 35 tested initially) and 6 ISSR primers gave 111
distinct bands in RAPD and 43 in ISSR, and exhibited uniform RAPD and ISSR
banding patterns for Ro plants tested. Our results suggested that present
protocol used for in vitro corm
formation may cost-effectively be applied for conservation of taro germplasm
along with maintaining the genetic stability and functionality of plants.
Further possibility may also be explored to use this protocol for production of
disease-free planting materials. This will facilitate the national and
international exchange of taro germplasms. |
| Page(s): | 535-542 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.05(4) [October 2006]
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