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Title: Micropropagation of <i style="">Embelia ribes </i>Burm.f. using inflorescence segments
Authors: Shankarmurthy, K
Krishna, V
Keywords: Embelia ribes
inflorescence culture
Issue Date: Oct-2006
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>8</sup> A01H4/00<b style=""></b>
Abstract: An efficient micropropagation protocol was developed for a threatened medicinal plant, <i style="">Embelia ribes</i> using inflorescence explants. The immature ovaries of the inflorescence segments proliferated into luxuriant mass of callus on Murashige and Skoog’s (MS) medium supplemented with IBA (3.5 mg/L) and Kn (0.5 mg/L). The combination of Kn and NAA in the range of 2.0 to 4.0 mg/L and 0.2 to 0.6 mg/L, respectively provoked the calli to differentiate into both shoot and root initials from the ovary callus. Highest number of regenerants (25±0.81per callus) were obtained at the concentration of 3.0 mg/L Kn and 0.4 mg/L NAA. The regenerants were transferred to the pots containing sterilized soil and hardened for a week. A mean of 21.82±1.02 regenerants per harvest were well acclimatized to the natural conditions exhibiting a normal development.
Description: 551-554
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(4) [October 2006]

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