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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(4) [October 2006] >
| Title: | Micropropagation of Embelia ribes Burm.f. using inflorescence segments |
| Authors: | Shankarmurthy, K
Krishna, V |
| Keywords: | Embelia ribes
inflorescence culture
micropropagation |
| Issue Date: | Oct-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 A01H4/00 |
| Abstract: | An efficient micropropagation protocol was
developed for a threatened medicinal plant, Embelia
ribes using inflorescence explants. The immature ovaries of the inflorescence
segments proliferated into luxuriant mass of callus on Murashige and Skoog’s
(MS) medium supplemented with IBA (3.5 mg/L) and Kn (0.5 mg/L). The combination
of Kn and NAA in the range of 2.0 to 4.0 mg/L and 0.2 to 0.6 mg/L, respectively
provoked the calli to differentiate into both shoot and root initials from the
ovary callus. Highest number of regenerants (25±0.81per callus) were obtained at the concentration of 3.0 mg/L Kn
and 0.4 mg/L NAA. The regenerants were transferred to the pots containing sterilized
soil and hardened for a week. A mean of 21.82±1.02 regenerants per harvest were well acclimatized to the natural
conditions exhibiting a normal development. |
| Page(s): | 551-554 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.05(4) [October 2006]
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