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IJBT Vol.05(4) [October 2006] >


Title: Micropropagation of Embelia ribes Burm.f. using inflorescence segments
Authors: Shankarmurthy, K
Krishna, V
Keywords: Embelia ribes
inflorescence culture
micropropagation
Issue Date: Oct-2006
Publisher: CSIR
IPC CodeInt. Cl.8 A01H4/00
Abstract: An efficient micropropagation protocol was developed for a threatened medicinal plant, Embelia ribes using inflorescence explants. The immature ovaries of the inflorescence segments proliferated into luxuriant mass of callus on Murashige and Skoog’s (MS) medium supplemented with IBA (3.5 mg/L) and Kn (0.5 mg/L). The combination of Kn and NAA in the range of 2.0 to 4.0 mg/L and 0.2 to 0.6 mg/L, respectively provoked the calli to differentiate into both shoot and root initials from the ovary callus. Highest number of regenerants (25±0.81per callus) were obtained at the concentration of 3.0 mg/L Kn and 0.4 mg/L NAA. The regenerants were transferred to the pots containing sterilized soil and hardened for a week. A mean of 21.82±1.02 regenerants per harvest were well acclimatized to the natural conditions exhibiting a normal development.
Page(s): 551-554
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.05(4) [October 2006]

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