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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(4) [October 2006] >
| Title: | Assessment of variability in the regenerants from long-term cultures of ‘safed musli’ (Chlorophytum borivilianum) |
| Authors: | Arora, Dilip K
Suri, Sarabjeet S
Ramawat, K G |
| Keywords: | Chlorohphytum borivilianum
RAPD
somaclonal variation
somatic embryos |
| Issue Date: | Oct-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 A01H4/00; C12N15/10 |
| Abstract: | Somatic embryogenesis in long-term calluses of seedling and leaf
explants, obtained from in vitro
grown plants of Chlorophytum
borivilianum—an endangered medicinal herb, has been achieved. Large number
of plants were established in the field and evaluated for variability among the
regenerants. The plantlets obtained through seedling-derived embryonic callus showed high level of morphological and
cytological variations, which increased with the increase in age of cultures.
Occasionally, variegated plants were also observed. Variation in leaf size,
stomatal number, epidermal cell size and chromosomal number was observed in
regenerants. Chromosomal variation was the least (3X-3 to 3X+3) in regenerants
from 1 to 4-month-old cultures and increased (5X-1 to 7X) with the age in
regenerants from cultures older than 6 months. Alternatively, regenerated
plants from somatic embryos recurrently obtained from leaf explants showed very
little variation (0.62%) in RAPD fingerprinting. However, further improvement
in somatic embryogenesis is required for domestication of the plant using
biotechnological method of propagation. |
| Page(s): | 527-534 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.05(4) [October 2006]
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