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Title: In vitro propagation of Ceropegia bulbosa using nodal segments
Authors: Goyal, Divya
Bhadauria, Seema
Keywords: in vitro propagation
Ceropegia bulbosa
threatened species
nodal explant
Issue Date: Oct-2006
Publisher: CSIR
Series/Report no.: Int. Cl.8 A01H4/00
Abstract: In vitro propagation of Ceropegia bulbosa, a threatened species has been achieved, which could be used to conserve it. Initially, nutrient media used were basal MS medium and MS media supplemented with cytokinins, BAP (2.22-8.87 µM/L) or kinetin (2.32-9.29 µM/L) or TDZ (2.27-9.08 µM/L) with combination of GA (0.58 µM/L) and NAA (0.27 µM/L) Nodal explants were used for establishing in vitro cultures. Mercuric chloride eliminated the contamination effectively from nodal explants after treating with Bavistin and Streptomycin with a good survival rate. One or two buds developed from a node after two weeks. BAP (4.44 µM/L) with GA (0.58 µM/L) and NAA (0.27 µM/L) showed highest frequency of formation of shoots (85%). Microshoots were transferred on MS medium supplemented with different concentrations of auxins IAA (2.85-11.42 µM/L) and NAA (2.69-10.74 µM/L) with BAP (3.55 µM/L) for rooting. IAA (11.42 µM/L) with BAP (3.55 µM/ L) showed highest rooting percentage. The rooted plantlets were acclimatized and hardened successfully within ten days of transferring them into plastic cups containing sterilized vermiculite for hardening.
Description: 565-567
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(4) [October 2006]

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