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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(4) [October 2006] >
| Title: | In vitro propagation of Ceropegia bulbosa using nodal segments |
| Authors: | Goyal, Divya
Bhadauria, Seema |
| Keywords: | in vitro propagation
Ceropegia bulbosa
threatened species
nodal explant |
| Issue Date: | Oct-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 A01H4/00 |
| Abstract: | In vitro propagation of Ceropegia bulbosa, a threatened species has been achieved, which
could be used to conserve it. Initially, nutrient media used were basal MS
medium and MS media supplemented with cytokinins, BAP (2.22-8.87 µM/L)
or kinetin (2.32-9.29 µM/L) or TDZ (2.27-9.08 µM/L)
with combination of GA (0.58 µM/L) and NAA (0.27 µM/L) Nodal explants were
used for establishing in vitro cultures.
Mercuric chloride eliminated the contamination effectively from nodal explants after treating with Bavistin and Streptomycin
with a good survival rate. One or two buds developed from a node after
two weeks. BAP (4.44 µM/L) with GA (0.58 µM/L) and NAA (0.27 µM/L)
showed highest frequency of formation of shoots (85%). Microshoots were
transferred on MS medium supplemented with different concentrations of auxins
IAA (2.85-11.42 µM/L) and NAA (2.69-10.74 µM/L)
with BAP (3.55 µM/L) for rooting. IAA (11.42 µM/L) with BAP (3.55 µM/ L) showed highest
rooting percentage. The rooted plantlets were acclimatized and hardened
successfully within ten days of transferring them into plastic cups containing
sterilized vermiculite for hardening. |
| Page(s): | 565-567 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.05(4) [October 2006]
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