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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(3) [July 2006] >
| Title: | Efficient Pseudomonas putida for degradation of p-nitrophenol |
| Authors: | Kulkarni, Meenal
Chaudhari, Ambalal |
| Keywords: | p-nitrophenol
biodegradation
Pseudomonas putida
hydroquinone
ring fission |
| Issue Date: | Jul-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 B09C1/10 |
| Abstract: | Exploration of Pseudomonas putida for controlling
environmental (p-nitrophenol) PNP
pollution revealed that 0.5 OD inoculum at 600 nm degraded 100 ppm of PNP
completely under the ambient conditions within 24 h as established by
UV-visible spectrophotometric and TLC analysis of treated broth and confirmed
the same by HPLC analysis. An attempt to elucidate the mechanism of PNP
degradation indicated that (i) it was oxidative with a stoichiometric release
of nitrite, (ii) PNP was converted to hydroquinone, which accumulated in the
presence of 2,2'-dipyridyl, (iii) hydroquinone was the
first product of ring oxygenation and nitrite removal, as confirmed by TLC and
HPLC analysis and (iv) concomitant formation of a keto acid by ring fission,
which was confirmed by Rothera's test. |
| Page(s): | 411-415 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.05(3) [July 2006]
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