NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(3) [July 2006] >


Title: Efficient Pseudomonas putida for degradation of p-nitrophenol
Authors: Kulkarni, Meenal
Chaudhari, Ambalal
Keywords: p-nitrophenol
biodegradation
Pseudomonas putida
hydroquinone
ring fission
Issue Date: Jul-2006
Publisher: CSIR
IPC CodeInt. Cl.7 B09C1/10
Abstract: Exploration of Pseudomonas putida for controlling environmental (p-nitrophenol) PNP pollution revealed that 0.5 OD inoculum at 600 nm degraded 100 ppm of PNP completely under the ambient conditions within 24 h as established by UV-visible spectrophotometric and TLC analysis of treated broth and confirmed the same by HPLC analysis. An attempt to elucidate the mechanism of PNP degradation indicated that (i) it was oxidative with a stoichiometric release of nitrite, (ii) PNP was converted to hydroquinone, which accumulated in the presence of 2,2'-dipyridyl, (iii) hydroquinone was the first product of ring oxygenation and nitrite removal, as confirmed by TLC and HPLC analysis and (iv) concomitant formation of a keto acid by ring fission, which was confirmed by Rothera's test.
Page(s): 411-415
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.05(3) [July 2006]

Files in This Item:

File Description SizeFormat
IJBT 5(3) 411-415.pdf238.54 kBAdobe PDFView/Open
 Current Page Visits: 574 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 603225 since 06-Feb-2009  Last updated on 21-Oct-2014Webmaster: nopr@niscair.res.in