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Title: Micropropagation of Azadirachta indica A. Juss. via cotyledonary nodes
Authors: Reddy, A Rajasekhar
Bavaji, M
Rao, J V S
Keywords: Azadirachta indica
cotyledonary node
micropropagation
Issue Date: Jul-2006
Publisher: CSIR
IPC CodeInt. Cl.8 A01H4/00
Abstract: An efficient protocol for micropropagation of Azadirachta indica A. Juss., a medicinally important plant, has been standardized. Cotyledonary nodes (1 cm long) excised from 15-20 d old in vitro-germinated seedlings were used as explants. The seeds were germinated on half strength MS medium devoid of phytohormones. Cotyledonary nodes were cultured on MS medium supplemented with different concentrations of cytokinins (BA/TDZ/2-ip) and auxins (2,4-D and NAA). Maximum shoot proliferation from single explant was obtained on MS media incorporated with TDZ (1.5 M), 2,4-D (0.5 M), adenine sulphate (40 mg/L), glutamine (100 mg/L) and thiamine HC1 (10 mg/L). In vitro-produced shoots were induced roots on half strength MS medium supplemented with a range of IBA concentrations (0.5-5.0 M). However, the highest frequency of root proliferation was observed on half strength MS medium supplemented with 2.0 M IBA. The regenerants were transferred to field conditions after acclimatization with a success rate of 80%
Page(s): 309-311
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.05(3) [July 2006]

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