Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/7761
Title: Micropropagation of <i style="">Azadirachta indica </i>A. Juss. via cotyledonary nodes
Authors: Reddy, A Rajasekhar
Bavaji, M
Rao, J V S
Keywords: <i style="">Azadirachta indica</i>
cotyledonary node
micropropagation
Issue Date: Jul-2006
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>8</sup> A01H4/00<b style=""></b>
Abstract: An efficient protocol for micropropagation of <i style="">Azadirachta indica </i>A. Juss., a medicinally important plant, has been standardized. Cotyledonary nodes (1 cm long) excised from 15-20 d old <i style="">in vitro-</i>germinated seedlings were used as explants. The seeds were germinated on half strength MS medium devoid of phytohormones. Cotyledonary nodes were cultured on MS medium supplemented with different concentrations of cytokinins (BA/TDZ/2-ip) and auxins (2,4-D and NAA). Maximum shoot proliferation from single explant was obtained on MS media incorporated with TDZ (1.5 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i>), 2,4-D (0.5 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i>), adenine sulphate (40 mg/L), glutamine (100 mg/L) and thiamine HC1 (10 mg/L). <i style="">In vitro-</i>produced shoots were induced roots on half strength MS medium supplemented with a range of IBA concentrations (0.5-5.0 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i>). However, the highest frequency of root proliferation was observed on half strength MS medium supplemented with 2.0 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> IBA. The regenerants were transferred to field conditions after acclimatization with a success rate of 80%
Description: 309-311
URI: http://hdl.handle.net/123456789/7761
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(3) [July 2006]

Files in This Item:
File Description SizeFormat 
IJBT 5(3) 309-311.pdf591.63 kBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.