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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(3) [July 2006] >
| Title: | Micropropagation of Azadirachta indica A. Juss. via cotyledonary nodes |
| Authors: | Reddy, A Rajasekhar
Bavaji, M
Rao, J V S |
| Keywords: | Azadirachta indica
cotyledonary node
micropropagation |
| Issue Date: | Jul-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 A01H4/00 |
| Abstract: | An efficient protocol for micropropagation of Azadirachta indica A. Juss., a
medicinally important plant, has been standardized. Cotyledonary nodes (1 cm
long) excised from 15-20 d old in vitro-germinated
seedlings were used as explants. The seeds were germinated on half strength MS
medium devoid of phytohormones. Cotyledonary nodes were cultured on MS medium
supplemented with different concentrations of cytokinins (BA/TDZ/2-ip) and
auxins (2,4-D and NAA). Maximum shoot proliferation from single explant was obtained
on MS media incorporated with TDZ (1.5  M), 2,4-D (0.5 M), adenine sulphate
(40 mg/L), glutamine (100 mg/L) and thiamine HC1 (10 mg/L). In vitro-produced shoots were induced
roots on half strength MS medium supplemented with a range of IBA concentrations
(0.5-5.0 M).
However, the highest frequency of root proliferation was observed on half
strength MS medium supplemented with 2.0 M IBA. The regenerants
were transferred to field conditions after acclimatization with a success rate
of 80% |
| Page(s): | 309-311 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.05(3) [July 2006]
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