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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(3) [July 2006] >
| Title: | Thidiazuron induced high frequency shoot bud formation and plant regeneration from cotyledonary node explants of Capsicum annuum L. |
| Authors: | Siddique, Iram
Anis, Mohammad |
| Keywords: | Capsicum annuum
cotyledonary node explants
plant regeneration
ex vitro rooting
relative water content |
| Issue Date: | Jul-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 A01H4/00, 5/04 |
| Abstract: | An efficient
protocol for rapid in vitro
propagation of Capsicum annuum L. cv.
Pusa jwala through multiple shoot bud formation from cotyledonary node explants
of 15-day-old aseptic seedlings has been developed. Multiple shoot bud
formation and highest rate of multiplication was standardized on Murashige and
Skoog (MS) medium supplemented with 1.5 µM
thidiazuron (TDZ) and 0.5 µM indole
3-acetic acid (IAA). The regenerated shoot buds when subcultured on hormone
free MS medium gave the highest rate of shoot proliferation and shoot length by
the end of third subculture. Individual elongated shoots were rooted best on MS
media containing 1.0 µM α-naphthalene
acetic acid (NAA). Ex vitro rooting
was also achieved when the basal cut ends of the in vitro regenerated shoots were dipped in 200 µM indole-3-butyric acid (IBA) for half
an hour followed by transplantation in plastic pots containing sterile garden
soil. Plantlets went through a hardening phase in a controlled plant growth
chamber, prior to field transfer. Of
all the micropropagated plants, about 95% survived following transfer to soil.
Growth performance of 2-month-old in
vitro raised plants was compared with in
vivo seedlings of the same age on the basis of selected morphological and
physiological parameters. Carotenoids and relative water content were
significantly higher in in vitro
regenerated plants as compared to in vivo
control plants of same age. |
| Page(s): | 303-308 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.05(3) [July 2006]
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