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Title: Thidiazuron induced high frequency shoot bud formation and plant regeneration from cotyledonary node explants of Capsicum annuum L.
Authors: Siddique, Iram
Anis, Mohammad
Keywords: Capsicum annuum
cotyledonary node explants
plant regeneration
ex vitro rooting
relative water content
Issue Date: Jul-2006
Publisher: CSIR
IPC CodeInt. Cl.8 A01H4/00, 5/04
Abstract: An efficient protocol for rapid in vitro propagation of Capsicum annuum L. cv. Pusa jwala through multiple shoot bud formation from cotyledonary node explants of 15-day-old aseptic seedlings has been developed. Multiple shoot bud formation and highest rate of multiplication was standardized on Murashige and Skoog (MS) medium supplemented with 1.5 µM thidiazuron (TDZ) and 0.5 µM indole 3-acetic acid (IAA). The regenerated shoot buds when subcultured on hormone free MS medium gave the highest rate of shoot proliferation and shoot length by the end of third subculture. Individual elongated shoots were rooted best on MS media containing 1.0 µM α-naphthalene acetic acid (NAA). Ex vitro rooting was also achieved when the basal cut ends of the in vitro regenerated shoots were dipped in 200 µM indole-3-butyric acid (IBA) for half an hour followed by transplantation in plastic pots containing sterile garden soil. Plantlets went through a hardening phase in a controlled plant growth chamber, prior to field transfer. Of all the micropropagated plants, about 95% survived following transfer to soil. Growth performance of 2-month-old in vitro raised plants was compared with in vivo seedlings of the same age on the basis of selected morphological and physiological parameters. Carotenoids and relative water content were significantly higher in in vitro regenerated plants as compared to in vivo control plants of same age.
Page(s): 303-308
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.05(3) [July 2006]

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