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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(2) [April 2006] >
| Title: | Development of RT-PCR based method for detection of potato virus Y in tobacco and potato |
| Authors: | Ghosh, S B
Bapat, V A |
| Keywords: | ELISA
NASH
potato virus Y (PVY)
RT-PCR |
| Issue Date: | Apr-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 C12N15/10 |
| Abstract: | A reverse transcription polymerase chain reaction (RT-PCR)
based protocol with a pair of potato virus Y coat protein gene specific primers
was found to be more sensitive method for the PVY detection in infected tobacco
leaf and potato tuber tissues than conventional enzyme linked immunosorbent
assay (ELISA) or nucleic acid spot hybridization (NASH) based methods. It was
found in our studies that by RT-PCR, the presence of the virus could be
detected in potato tubers of two field samples, which could not be detected by
NASH or ELISA. |
| Page(s): | 232-235 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.05(2) [April 2006]
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