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IJBT Vol.05(2) [April 2006] >


Title: Development of RT-PCR based method for detection of potato virus Y in tobacco and potato
Authors: Ghosh, S B
Bapat, V A
Keywords: ELISA
NASH
potato virus Y (PVY)
RT-PCR
Issue Date: Apr-2006
Publisher: CSIR
IPC CodeInt. Cl.8 C12N15/10
Abstract: A reverse transcription polymerase chain reaction (RT-PCR) based protocol with a pair of potato virus Y coat protein gene specific primers was found to be more sensitive method for the PVY detection in infected tobacco leaf and potato tuber tissues than conventional enzyme linked immunosorbent assay (ELISA) or nucleic acid spot hybridization (NASH) based methods. It was found in our studies that by RT-PCR, the presence of the virus could be detected in potato tubers of two field samples, which could not be detected by NASH or ELISA.
Page(s): 232-235
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.05(2) [April 2006]

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