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IJBT Vol.05 [2006] >
IJBT Vol.05(2) [April 2006] >
| Title: | Effect of different factors on non-symbiotic seed germination, formation of protocorm-like bodies and plantlet morphology of Cleisostoma racemiferum (Lindl.) Garay |
| Authors: | Temjensangba
Deb, Chitta Ranjan |
| Keywords: | Cleisostoma racemiferum
green pod culture
plant growth regulators
seed age |
| Issue Date: | Apr-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 A01H4/00 |
| Abstract: | Cleisostoma racemiferum, an epiphytic
orchid of primary forest under threat in their natural habitat, was studied.
Immature seeds of different developmental stages [(8-20 week after pollination
(WAP)] were cultured on Knudson ‘C’, Mitra et
al and MS media supplemented with sucrose (0-3%, w/v), coconut
water (CW, 0-20%, v/v) and NAA (0-30  M) + BA (0-8 M) singly or in combination. After 7 week of culture, first sign of
germination was recorded as nodular swelling of seeds. Amongst the three
different basal media tested, better germination was supported by MS medium,
followed by Mitra et al and Knudson
‘C’ media containing sucrose (3%) and NAA (10.0 M ) + BA (8.0 M). Of various
developmental stages of the seed, better germination was obtained from green
pods of 16 week after pollination. Younger seeds did not show any sign of
germination, while mature seeds exhibited delayed and deformed germination.
Though incorporation of CW in the medium did not show much influence on seed
germination, but 15% (v/v) CW in the initiation medium enhanced the early
differentiation of protocorm-like bodies (PLBs) into plantlets. Within 14-16
week of culture on germination medium, the PLBs started releasing the first set
of leaflets. The advanced stage PLBs were converted into rooted plantlets on MS
medium containing IAA (10.0 M) + Kn (9.0 M). Although the medium containing NAA (10.0 M) + BA (8.0 M) resulted in multiple
shoot buds, but the leaves were thin and etiolated. Further, the medium
enriched with NAA (10.0 M) + Kn (9.0 M) resulted in stunted growth of plantlets, while presence of IAA
(10.0 M) + BA (8.0 M) resulted in plantlets
with poor roots. |
| Page(s): | 223-228 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.05(2) [April 2006]
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