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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(2) [April 2006] >
| Title: | Somatic embryogenesis and plant regeneration in Soybean [Glycine max (L.) Merr.] |
| Authors: | Kumari, B D Ranjitha
Settu, A
Sujatha, G |
| Keywords: | embryonic axes
callus
somatic embryogenesis
plant regeneration
Glycine max |
| Issue Date: | Apr-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 A01H4/00 |
| Abstract: | Establishment of
efficient protocol for the induction of somatic embryogenesis and plant
regeneration from embryonic axes explants was achieved in Glycine max (L.) Merr. Highest frequency (92.9%) of embryogenic
callus formation was obtained from embryonic axes explants of cv. CO-1 on MS
media containing 2,4-Dichlorophenoxy acetic acid (2,4-D) and 6-benzyl adenine
(BA). Fast growing, yellowish nodular callus lines containing somatic embryos
were established on initiation medium supplemented with 180.8 µM 2,4-D and 2.22 µM BA, respectively. Embryo development and maturation was achieved
on MS medium supplemented with 2.26 µM
2,4-D and 8.88 µM BA. The well formed
embryos germinated into complete plantlets on MS medium containing 8.88 µM BA and 1.34 µM NAA (a-Naphthalene acetic acid). The regenerated
plants were first transferred to plastic cups and later into pots for
maturation. Regenerated plants did not exhibit observable morphological
alterations. |
| Page(s): | 243-245 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.05(2) [April 2006]
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