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Title: Somatic embryogenesis and plant regeneration in Soybean [Glycine max (L.) Merr.]
Authors: Kumari, B D Ranjitha
Settu, A
Sujatha, G
Keywords: embryonic axes
somatic embryogenesis
plant regeneration
Glycine max
Issue Date: Apr-2006
Publisher: CSIR
IPC CodeInt. Cl.8 A01H4/00
Abstract: Establishment of efficient protocol for the induction of somatic embryogenesis and plant regeneration from embryonic axes explants was achieved in Glycine max (L.) Merr. Highest frequency (92.9%) of embryogenic callus formation was obtained from embryonic axes explants of cv. CO-1 on MS media containing 2,4-Dichlorophenoxy acetic acid (2,4-D) and 6-benzyl adenine (BA). Fast growing, yellowish nodular callus lines containing somatic embryos were established on initiation medium supplemented with 180.8 µM 2,4-D and 2.22 µM BA, respectively. Embryo development and maturation was achieved on MS medium supplemented with 2.26 µM 2,4-D and 8.88 µM BA. The well formed embryos germinated into complete plantlets on MS medium containing 8.88 µM BA and 1.34 µM NAA (a-Naphthalene acetic acid). The regenerated plants were first transferred to plastic cups and later into pots for maturation. Regenerated plants did not exhibit observable morphological alterations.
Page(s): 243-245
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.05(2) [April 2006]

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