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|Title:||Somatic embryogenesis and plant regeneration in Soybean [<i style="">Glycine max</i> (L.) Merr.]|
|Authors:||Kumari, B D Ranjitha|
|Series/Report no.:||Int. Cl.<sup>8</sup> A01H4/00|
|Abstract:||Establishment of efficient protocol for the induction of somatic embryogenesis and plant regeneration from embryonic axes explants was achieved in <i style="">Glycine max </i>(L.) Merr. Highest frequency (92.9%) of embryogenic callus formation was obtained from embryonic axes explants of cv. CO-1 on MS media containing 2,4-Dichlorophenoxy acetic acid (2,4-D) and 6-benzyl adenine (BA). Fast growing, yellowish nodular callus lines containing somatic embryos were established on initiation medium supplemented with 180.8 µ<i style="">M</i> 2,4-D and 2.22 µ<i style="">M</i> BA, respectively. Embryo development and maturation was achieved on MS medium supplemented with 2.26 µ<i style="">M</i> 2,4-D and 8.88 µ<i style="">M</i> BA. The well formed embryos germinated into complete plantlets on MS medium containing 8.88 µ<i style="">M</i> BA and 1.34 µ<i style="">M</i> NAA (a-Naphthalene acetic acid). The regenerated plants were first transferred to plastic cups and later into pots for maturation. Regenerated plants did not exhibit observable morphological alterations.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.05(2) [April 2006]|
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