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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(2) [April 2006] >
| Title: | Rapid and recurrent in vitro mass-multiplication of androgenic rice embryos |
| Authors: | Roy, Bidhan
Mandal, Asit B |
| Keywords: | anther culture
microtillering
Oryza sativa
IR72 |
| Issue Date: | Apr-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 A01H4/00 |
| Abstract: | In
vitro mass multiplication of plantlets of androgenic origin was achieved in
an indica rice var. IR72. The
multiple shoots were developed profusely on MS medium containing 4 mg L-1
Kn. When the shoots were cultured on MS supplemented with 6 mg L-1
BAP, a large number of dormant embryos, pro-embryos and embryo-like structures
were produced. Those multiple shoots and dormant embryos had no roots. The
subcultured shoots and the dormant embryos on MS basal developed into healthy
plantlets with prolific roots. Since 1998, these dormant embryos and the shoots
are being sub-cultured at 45 d interval. Till date the multiple shoots and the
dormant embryos are developing constantly depending upon the hormones used in
the medium. The stock is being maintained by recurrent subculture using
explants from the preceding culture. Such stock offers ample scope in genetic
transformation of indica rice and
multiplication of transgenic plantlets. The protocol also prospects efficient in vitro conservation through repeated
subculture. It provides a novel source too for constant supply of embryos to
produce synthetic seeds of androgenic origin in rice. |
| Page(s): | 239-242 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.05(2) [April 2006]
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