NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(1) [January 2006] >


Title: Occurrence of Iris mild mosaic potyvirus in cultivated iris in India
Authors: Kulshrestha, Saurabh
Hallan, Vipin
Raikhy, Gaurav
Ram, Raja
Garg, I D
Haq, Q M R
Zaidi, A A
Keywords: cytopathology
ELISA
IC-RT-PCR
IEM
Iris mild mosaic potyvirus (IMMV)
Issue Date: Jan-2006
Publisher: CSIR
IPC CodeInt. Cl.7 C07K16/10; C12N15/10; G01N33/53
Abstract: Iris (Iris x hollandica Hort. cv. Bluemagic) plants showing mosaic symptoms were tested for the presence of Iris mild mosaic potyvirus (IMMV) by host range, enzyme linked immunosorbent assay (ELISA) using antibodies specific for IMMV), reverse transcription polymerase chain reaction (RT-PCR), immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) using potyvirus group specific primers and antibodies specific to IMMV, immune electron microscopy and cytopathology. Presence of IMMV was detected by ELISA, confirmed by RT-PCR, IC-RT-PCR, IEM, cytopathology and sequencing of the cloned PCR product. From the sequence of the virus, specific primers were designed and specific detection of IMMV from iris was standardized.
Page(s): 94-98
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.05(1) [January 2006]

Files in This Item:

File Description SizeFormat
IJBT 5(1) 94-98.pdf452.23 kBAdobe PDFView/Open
 Current Page Visits: 625 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 555947 since 06-Feb-2009  Last updated on 15-Jul-2014Webmaster: nopr@niscair.res.in