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Title: Occurrence of <i style="">Iris mild mosaic potyvirus</i> in cultivated iris in India
Authors: Kulshrestha, Saurabh
Hallan, Vipin
Raikhy, Gaurav
Ram, Raja
Garg, I D
Haq, Q M R
Zaidi, A A
Keywords: cytopathology
<i style="">Iris mild mosaic potyvirus</i> (IMMV)
Issue Date: Jan-2006
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>7</sup> C07K16/10; C12N15/10; G01N33/53
Abstract: Iris (<i style="">Iris</i> x<i style=""> hollandica</i> Hort. cv. Bluemagic) plants showing mosaic symptoms were tested for the presence of <i style="">Iris mild mosaic potyvirus</i> (IMMV) by host range, enzyme linked immunosorbent assay (ELISA) using antibodies specific for IMMV), reverse transcription polymerase chain reaction (RT-PCR), immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) using potyvirus group specific primers and antibodies specific to IMMV, immune electron microscopy and cytopathology. Presence of IMMV was detected by ELISA, confirmed by RT-PCR, IC-RT-PCR, IEM, cytopathology and sequencing of the cloned PCR product. From the sequence of the virus, specific primers were designed and specific detection of IMMV from iris was standardized.
Description: 94-98
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(1) [January 2006]

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