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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(1) [January 2006] >
| Title: | Stable integration, expression and inheritance of the ferritin gene in transgenic elite indica rice cultivar BR29 with enhanced iron level in the endosperm |
| Authors: | Khalekuzzaman, M
Datta, K
Oliva, N
Alam, M F
Joarder, 0 I
Datta, S K |
| Keywords: | indica rice BR29
inheritance
iron accumulation
nutrition
transgenic rice |
| Issue Date: | Jan-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 C12N15/09, 15/29 |
| Abstract: | Rice (Oryza sativa L.) is a major
crop providing staple diet for more than half of the world's population, but it
does not
fulfill the recommended daily dietary allowance.
Moreover, milling of rice grain causes considerable losses of nutrients,including
iron. Iron deficiency is a global nutritional problem. About 3.5 billion people
in the developing world suffer from iron-deficiency anemia, of which 50% is
dietary in origin. To increase iron storage in rice, we introduced the ferritin gene driven by an endosperm-specific glutelin promoter
into a Bangladeshi rice cultivar, BRRl Dhan 29 (BR29), using the biolistic
method. Analysis demonstrated integration, inheritance and expression of the ferritin gene up to the T3 generation.The
iron content in seeds was estimated by using the ICP (Inductively Coupled Argon
Plasma) Spectrometer. All transgenic plants accumulated higher levels of iron
in the grain, with as much as 9.2 mg/kg versus the control (3.8 mg/kg). A histochemical reaction of the thin microtome section
revealed the presence of iron in the endosperm cells of the transgenic grain.
This finding suggests that homozygous rice lines with enhanced iron content
developed by genetic engineering could help overcome iron deficiency in
developing countries. |
| Page(s): | 26-31 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.05(1) [January 2006]
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