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Title: In vitro flowering and rapid propagation of Vitex negundo L.—A medicinal plant
Authors: Vadawale, A V
Barve, D M
Dave, A M
Keywords: inflorescence;medicinal plant;micropropagation;Verbenaceae;Vitex negundo
Issue Date: Jan-2006
Publisher: CSIR
IPC Code: Int. Cl.7 A01H4/00, 5/02
Abstract: In vitro flowering and an efficient micropropagation protocol was developed for the woody, aromatic and medicinal shrub Vitex negundo (Verbenaceae) by the in vitro culture of nodal segments of mature plant. Murashige and Skoog’s (MS) medium supplemented with 4.4 M N6-benzylaminopurine (BAP) and 0.53 M α-naphthaleneacetic acid (NAA) were found to develop fully functional flowers. MS medium supplemented with 4.4 M BAP and 0.53 M NAA induced an average of five shoots per node and was the best for axillary bud proliferation. Subsequent cultures enhanced the number of shoots. Full strength MS solid medium with 3.69 M indole-3-butyric acid (IBA) exhibited the best in vitro rooting. Ninety per cent of the rooted shoots survived when transferred to green house and subsequently to the field.
Page(s): 112-116
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(1) [January 2006]

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