Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/7748
Title: <i style="">In vitro</i> flowering and rapid propagation of <i style="">Vitex negundo </i>L<i style="">.</i>—A medicinal plant
Authors: Vadawale, A V
Barve, D M
Dave, A M
Keywords: inflorescence
medicinal plant
micropropagation
Verbenaceae
<i style="">Vitex negundo</i>
Issue Date: Jan-2006
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>7</sup> A01H4/00, 5/02
Abstract: <i style="">In vitro </i>flowering and an efficient micropropagation protocol was developed for the woody, aromatic and medicinal shrub <i style="">Vitex negundo</i> (Verbenaceae) by the <i style="">in vitro</i> culture of nodal segments of mature plant. Murashige and Skoog’s (MS) medium supplemented with 4.4 <img src='/image/spc_char/micro.gif' border=0><i style="">M</i> N<sup>6</sup>-benzylaminopurine (BAP) and 0.53 <img src='/image/spc_char/micro.gif' border=0><i style="">M</i> α-naphthaleneacetic acid (NAA) were found to develop fully functional flowers. MS medium supplemented with 4.4 <img src='/image/spc_char/micro.gif' border=0><i style="">M</i> BAP and 0.53 <img src='/image/spc_char/micro.gif' border=0><i style="">M</i> NAA induced an average of five shoots per node and was the best for axillary bud proliferation. Subsequent cultures enhanced the number of shoots. Full strength MS solid medium with 3.69 <img src='/image/spc_char/micro.gif' border=0><i style="">M </i>indole-3-butyric acid (IBA) exhibited the best <i style="">in vitro </i>rooting. Ninety per cent of the rooted shoots survived when transferred to green house and subsequently to the field.
Description: 112-116
URI: http://hdl.handle.net/123456789/7748
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(1) [January 2006]

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