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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(1) [January 2006] >
| Title: | In vitro flowering and rapid propagation of Vitex negundo L.—A medicinal plant |
| Authors: | Vadawale, A V
Barve, D M
Dave, A M |
| Keywords: | inflorescence
medicinal plant
micropropagation
Verbenaceae
Vitex negundo |
| Issue Date: | Jan-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 A01H4/00, 5/02 |
| Abstract: | In vitro flowering and an
efficient micropropagation protocol was developed for the woody, aromatic and
medicinal shrub Vitex negundo
(Verbenaceae) by the in vitro culture
of nodal segments of mature plant. Murashige and Skoog’s (MS) medium
supplemented with 4.4  M N6-benzylaminopurine
(BAP) and 0.53 M α-naphthaleneacetic acid (NAA) were found to develop fully
functional flowers. MS medium supplemented with 4.4 M BAP and 0.53 M NAA induced an average of
five shoots per node and was the best for axillary bud proliferation.
Subsequent cultures enhanced the number of shoots. Full strength MS solid
medium with 3.69 M
indole-3-butyric acid (IBA) exhibited the best in vitro rooting. Ninety per cent of the
rooted shoots survived when transferred to green house and subsequently to the
field. |
| Page(s): | 112-116 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.05(1) [January 2006]
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