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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.05 [2006] >
IJBT Vol.05(1) [January 2006] >
| Title: | Genotype dependent influence of phytohormone combination and subculturing on micropropagation of sugarcane varieties |
| Authors: | Singh, Nandita
Kumar, Anil
Garg, G K |
| Keywords: | genotype
micropropagation
phytohormone combinations
Saccharum |
| Issue Date: | Jan-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 A01H4/00 |
| Abstract: | Eight
varieties of sugarcane, viz. Co.89003, Co.91010, Co.96258, Co.97017, Co.P84211,
Co.P84212, Co.P90223, and Co.S767, were successfully established and micropropagated
using shoots tips as explants. Four combinations of phytohormone, having NAA (1
mg/L) at fixed conc. and two cytokinins, i.e. BAP and Kn, at varying conc.,
were used; these were designated as A1 (1 mg/L BAP + 2 mg/L Kn); A2
(1 mg/L BAP + 3 mg/L Kn); A3 (3 mg/L BAP + 2 mg/L Kn) and A4
(6 mg/L BAP and 1 mg/L Kn). All the varieties were successively sub cultured up
to 8th passage. Maximum percentage of explant establishment
(80-100%) was observed for all the varieties, except Co.P.84212 and Co.P. 90223
(50-60%). No influence of genotype was found on the days required for side bud
induction, average length of shoots from side bud, and number of days for
multiple shoots when explants were placed in various phytohormone combinations.
However, individual varieties showed preference for higher or lower conc. of
cytokinins, i.e. growth of shoots in varieties Co.89003, Co.91010, Co.96258,
Co.97017, Co.P84212 and Co.P.90223 was better in A1 and A2
hormone profile, while that of Co.91010 and Co.S767 in A3 and A4
hormone profile. |
| Page(s): | 99-106 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.05(1) [January 2006]
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