NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(3) [July 2005] >


Title: Cloning, characterization and expression of bovine (Bos indicus) tumour necrosis factor-
Authors: Gupta, Praveen K
Bind, R B
Walunj, Sameer S
Saini, Mohini
Keywords:  Bos indicus
cDNA sequence
expression
TNF-
Issue Date: Jul-2005
Publisher: CSIR
IPC CodeInt. Cl.7 C12N15/10, 15/28
Abstract: The gene for tumour necrosis factor-alpha (TNF- ) was amplified from cDNA pool prepared from LPS-stimulated bovine peripheral blood mononuclear cells isolated from Indian cattle. The amplified TNF- gene was cloned and nucleotide sequences were determined. Homology comparison of nucleotide and predicted amino acid sequences revealed similarity at nucleotide and amino acid level in both exotic cattle and goat. The coding sequence of mature TNF- (without its signal sequence and transmembrane anchor) was expressed as fusion protein with N-terminal polyhistidine using prokaryotic expression vector. The expressed protein was present as insoluble inclusion bodies. The recombinant protein was solubilized with urea and purified using Ni-agarose affinity chromatography. The purified recombinant TNF- was characterized in SDS-PAGE and in western blotting.
Page(s): 363-366
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.04(3) [July 2005]

Files in This Item:

File Description SizeFormat
IJBT 4(3) 363-366.pdf389.76 kBAdobe PDFView/Open
 Current Page Visits: 816 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 606327 since 06-Feb-2009  Last updated on 21-Oct-2014Webmaster: nopr@niscair.res.in