NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(2) [April 2005] >

Title: In vitro regeneration and mass multiplication of Psoralea corylifolia—An endangered medicinal plant
Authors: Anis, Mohammad
Faisal, Mohd
Keywords: endangered
in vitro plant regeneration
MS medium
Psoralea corylifolia
tissue culture
Issue Date: Apr-2005
Publisher: CSIR
IPC CodeInt. cl.7 A01H4/00,5/00
Abstract: An efficient protocol has been developed for plant regeneration from shoot tip and nodal explants of in vitro grown Psoralea corylifolia. Nodal segments were more morphogenic to shoot bud differentiation than shoot tips. Proliferation of shoots was achieved on Murashige and Skoog (MS) medium supplemented with various concentrations of BA, Kn, IAA, NAA either singly or in various combinations. The highest shoot regeneration frequency (90%) and number of shoots (12.0 ± 0.57) were obtained from nodal segment on MS medium fortified with 5 µM BA and 0.5 µM NAA. Addition of CH (100 mg/l) to the shoot induction medium enhanced the growth of regenerants. The regenerated shoots rooted best on MS medium containing 0.5 µM IBA. Regenerated plantlets with well-developed shoot and roots were hardened, successfully transferred to soil and maintained in green house. The present in vitro procedure can be used in conservation and mass propagation of this endangered medicinal plant.
Page(s): 261-264
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.04(2) [April 2005]

Files in This Item:

File Description SizeFormat
IJBT 4(2) 261-264.pdf299.99 kBAdobe PDFView/Open
 Current Page Visits: 1094 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 163555 since 01-Sep-2015  Last updated on 21-Jun-2016Webmaster: