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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(2) [April 2005] >
| Title: | In vitro regeneration and mass multiplication of Psoralea corylifolia—An endangered medicinal plant |
| Authors: | Anis, Mohammad
Faisal, Mohd |
| Keywords: | endangered
in vitro plant regeneration
MS medium
Psoralea corylifolia
tissue culture |
| Issue Date: | Apr-2005 |
| Publisher: | CSIR |
| IPC Code: | Int. cl.7 A01H4/00,5/00 |
| Abstract: | An efficient
protocol has been developed for plant regeneration from shoot tip and nodal
explants of in vitro grown Psoralea corylifolia. Nodal segments
were more morphogenic to shoot bud differentiation than shoot tips.
Proliferation of shoots was achieved on Murashige and Skoog (MS) medium
supplemented with various concentrations of BA, Kn, IAA, NAA either singly or
in various combinations. The highest shoot regeneration frequency (90%) and
number of shoots (12.0 ± 0.57) were obtained from nodal segment on MS medium
fortified with 5 µM BA and 0.5 µM NAA. Addition of CH (100 mg/l) to the
shoot induction medium enhanced the growth of regenerants. The regenerated
shoots rooted best on MS medium containing 0.5 µM IBA. Regenerated plantlets with well-developed shoot and roots
were hardened, successfully transferred to soil and maintained in green house.
The present in vitro procedure can be
used in conservation and mass propagation of this endangered medicinal plant. |
| Page(s): | 261-264 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.04(2) [April 2005]
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