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IJBT Vol.04(2) [April 2005] >


Title: In vitro regeneration and mass multiplication of Psoralea corylifolia—An endangered medicinal plant
Authors: Anis, Mohammad
Faisal, Mohd
Keywords: endangered
in vitro plant regeneration
MS medium
Psoralea corylifolia
tissue culture
Issue Date: Apr-2005
Publisher: CSIR
IPC CodeInt. cl.7 A01H4/00,5/00
Abstract: An efficient protocol has been developed for plant regeneration from shoot tip and nodal explants of in vitro grown Psoralea corylifolia. Nodal segments were more morphogenic to shoot bud differentiation than shoot tips. Proliferation of shoots was achieved on Murashige and Skoog (MS) medium supplemented with various concentrations of BA, Kn, IAA, NAA either singly or in various combinations. The highest shoot regeneration frequency (90%) and number of shoots (12.0 ± 0.57) were obtained from nodal segment on MS medium fortified with 5 µM BA and 0.5 µM NAA. Addition of CH (100 mg/l) to the shoot induction medium enhanced the growth of regenerants. The regenerated shoots rooted best on MS medium containing 0.5 µM IBA. Regenerated plantlets with well-developed shoot and roots were hardened, successfully transferred to soil and maintained in green house. The present in vitro procedure can be used in conservation and mass propagation of this endangered medicinal plant.
Page(s): 261-264
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.04(2) [April 2005]

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