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Title: Characterization of β-galactosidase from an Antarctic <i>Bacillus</i> sp<i>.</i>
Authors: Dhaked, Ram Kumar
Alam, Syed Imteyaz
Singh, Lokendra
Keywords: Bacillus sp
<img src='/image/spc_char/beta.gif' border=0>-galactosidase-galactosidase
cold-active enzymes
Issue Date: Apr-2005
Publisher: CSIR
Series/Report no.: <b> Int.Cl.<sup>7</sup></b>: A01N63/02; C12N9/38; C12R1:07
Abstract: <smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"> Antarctica is the coldest continent on the earth and harbours a variety of microorganisms. One of the bacterial isolates from cyanobacterial mats of Schirmacher Oasis, characterized as <i>Bacillus </i>sp. grew at 5-35<sup>o</sup>C with an optima at 25<sup>o</sup>C, and produced intracellular cold active <img src='/image/spc_char/beta.gif' border=0>-galactosidase. The maximum activity was recorded at <i>p</i>H 6.8 and 40<sup>o</sup>C during late stationary phase. At 5<sup>o</sup>C, the enzyme retained 39.7% activity and at 60<sup>o</sup>C, became completely inactive within 15 min. The enzyme activity was stimulated by metal ions but was inhibited by ethylene diamine tetra acetic acid. Non-denaturing polyacylamide separation followed by <i>in situ</i> hydrolysis of 5-bromo-4-chloro-3-indolyl-<img src='/image/spc_char/beta.gif' border=0>-galactopyanoside, suggested the presence of isozymes. These properties of <img src='/image/spc_char/beta.gif' border=0>-galactosidase, indicate its potential use in removal of lactose from the milk for lactose intolerant people. </smarttagtype>
Description: 227-231
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.04(2) [April 2005]

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