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Title: Characterization of β-galactosidase from an Antarctic Bacillus sp.
Authors: Dhaked, Ram Kumar
Alam, Syed Imteyaz
Singh, Lokendra
Keywords: Bacillus sp
cold-active enzymes
Issue Date: Apr-2005
Publisher: CSIR
IPC Code Int.Cl.7: A01N63/02; C12N9/38; C12R1:07
Abstract:  Antarctica is the coldest continent on the earth and harbours a variety of microorganisms. One of the bacterial isolates from cyanobacterial mats of Schirmacher Oasis, characterized as Bacillus sp. grew at 5-35oC with an optima at 25oC, and produced intracellular cold active -galactosidase. The maximum activity was recorded at pH 6.8 and 40oC during late stationary phase. At 5oC, the enzyme retained 39.7% activity and at 60oC, became completely inactive within 15 min. The enzyme activity was stimulated by metal ions but was inhibited by ethylene diamine tetra acetic acid. Non-denaturing polyacylamide separation followed by in situ hydrolysis of 5-bromo-4-chloro-3-indolyl--galactopyanoside, suggested the presence of isozymes. These properties of -galactosidase, indicate its potential use in removal of lactose from the milk for lactose intolerant people.
Page(s): 227-231
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.04(2) [April 2005]

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