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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(2) [April 2005] >
| Title: | Differentiation of rabies fixed and street viruses using RT-PCR coupled with restriction endonuclease analysis |
| Authors: | Gupta, Praveen K
Chaturvedi, V K
Verma, P C
Pandey, K D |
| Keywords: | rabies
virus
differentiation
street
fixed
RT-PCR
restriction endonuclease analysis |
| Issue Date: | Apr-2005 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 C12N15/10, 15/47; C12R1:93 |
| Abstract: | A method based on RT-PCR and restriction
endonuclease digestion of amplified PCR product was used to differentiate
rabies laboratory “fixed” and “street” viruses. The primer sets from
glycoprotein and nucleoprotein gene of rabies virus were used to amplify 406
and 533 bp amplicons, respectively. After amplification in RT-PCR, the PCR
product was digested with HaeIII
restriction endonuclease and analysed for the presence or absence of
restriction site on amplicons. The presence of HaeIII restriction site on amplicons indicated rabies fixed virus
while absence indicated street virus. |
| Page(s): | 284-286 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.04(2) [April 2005]
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