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IJBT Vol.04(2) [April 2005] >


Title: Differentiation of rabies fixed and street viruses using RT-PCR coupled with restriction endonuclease analysis
Authors: Gupta, Praveen K
Chaturvedi, V K
Verma, P C
Pandey, K D
Keywords: rabies
virus
differentiation
street
fixed
RT-PCR
restriction endonuclease analysis
Issue Date: Apr-2005
Publisher: CSIR
IPC CodeInt. Cl.7 C12N15/10, 15/47; C12R1:93
Abstract: A method based on RT-PCR and restriction endonuclease digestion of amplified PCR product was used to differentiate rabies laboratory “fixed” and “street” viruses. The primer sets from glycoprotein and nucleoprotein gene of rabies virus were used to amplify 406 and 533 bp amplicons, respectively. After amplification in RT-PCR, the PCR product was digested with HaeIII restriction endonuclease and analysed for the presence or absence of restriction site on amplicons. The presence of HaeIII restriction site on amplicons indicated rabies fixed virus while absence indicated street virus.
Page(s): 284-286
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.04(2) [April 2005]

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