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Title:  Micropropagation and ecorestoration of Decalepis arayalpathra (Joseph & Chandra.) Venter—An endemic and endangered ethnomedicinal plant of Western Ghats
Authors: Gangaprasad, A
Decruse, S William
Seeni, S
Nair, G M
Keywords:  Decalepis arayalpathra;ecorestoration;endemic;micropropagation;Western Ghats
Issue Date: Apr-2005
Publisher: CSIR
IPC Code: Int Cl.7 A01H4/00, 5/00
Abstract: Single nodes from young top shoots of Decalepis arayalpathra were cultured on Murashige and Skoog’s (MS) agar medium supplemented with 0.1 to 5.0 mg l-1 BAP. All the concentrations of BAP induced single axillary shoot of varying length. However, MS medium supplemented with 1.0 mg l-1 BAP supported rapid growth and produced the longest shoots (6.8 cm) in 60 days. For further multiplication, the nodes and shoot tips from in vitro derived shoots were recultured on MS medium fortified with 0.5 mg l-1 BAP, which produced the 8 cm long shoots having 5-7 nodes in 30 days period. Further, the top microshoot cuttings (3-5 cm long) with 2-3 nodes, subcultured onto MS medium supplemented with 1.5 mg l-1 IAA, produced an average of 6.3 roots in 30 days period. The rooted plants after hardening were reintroduced into their natural habitat at Kallar reserve forest, Thiruvananthapuram. After two years, 84% survival of reintroduced plants was recorded.
Page(s): 265-270
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.04(2) [April 2005]

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