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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(2) [April 2005] >
| Title: | Micropropagation and ecorestoration of Decalepis arayalpathra (Joseph & Chandra.) Venter—An endemic and endangered ethnomedicinal plant of Western Ghats |
| Authors: | Gangaprasad, A
Decruse, S William
Seeni, S
Nair, G M |
| Keywords: | Decalepis arayalpathra
ecorestoration
endemic
micropropagation
Western Ghats |
| Issue Date: | Apr-2005 |
| Publisher: | CSIR |
| IPC Code: | Int Cl.7 A01H4/00, 5/00 |
| Abstract: | Single nodes from young top shoots of Decalepis arayalpathra were cultured on Murashige and Skoog’s (MS) agar
medium supplemented with 0.1 to 5.0 mg l-1 BAP. All the
concentrations of BAP induced single axillary shoot of varying length. However,
MS medium supplemented with 1.0 mg l-1 BAP supported rapid growth
and produced the longest shoots (6.8 cm) in 60 days. For further
multiplication, the nodes and shoot tips from in vitro derived shoots were recultured on MS medium fortified with
0.5 mg l-1 BAP, which produced the 8 cm long shoots having 5-7 nodes
in 30 days period. Further, the top microshoot cuttings (3-5 cm long) with 2-3
nodes, subcultured onto MS medium supplemented with 1.5 mg l-1 IAA,
produced an average of 6.3 roots in 30 days period. The rooted plants after
hardening were reintroduced into their natural habitat at Kallar reserve
forest, Thiruvananthapuram. After two years, 84% survival of reintroduced
plants was recorded. |
| Page(s): | 265-270 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.04(2) [April 2005]
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