Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/7732
Title: Plantlet regeneration via adventitious shoot bud proliferation from leaf explants in <i style="">Potentilla fulgens</i> Wall. ex Hook.—A plant possessing hypoglycemic activity
Authors: Laskar, M A
Lyngdoh, J P
Buam, J J
Syiem, D
Keywords: adventitious shoot regeneration
explant browning
<i style="">Potentilla fulgens</i>
Issue Date: Apr-2005
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>7</sup> A01H4/00, 5/00
Abstract: A method for adventitious shoot bud regeneration from leaves of <i style="">Potentilla fulgens</i> has been developed. Leaves were obtained from plants growing in the natural habitat. Explant browning, a major hurdle in the establishment of cultures, was overcome by treating leaves with a combination of antioxidants (100 mg l<sup>-1</sup> ascorbic acid, 100 mg l<sup>-1</sup> citric acid and 20 mg l<sup>-1</sup> L-cysteine HCl). Influence of the growth regulators BAP (6-benzylaminopurine) and NAA ( <img src='/image/spc_char/alpha.gif' border=0> -naphthaleneacetic acid) on adventitious bud differentiation and shoot regeneration was observed on modified Murashige and Skoog’s (MMS) agar medium. The most effective treatment was MMS with 0.1 mg l<sup>-1</sup> BAP and 0.1 mg l<sup>-1</sup> NAA, which gave 80% bud induction frequency with 38.4 BFC (Bud Forming Capacity) index and 48 shoots per explant of 3.5 cm length. Rooting was induced on MS basal medium. The regenerated plants had 70% survival rate.
Description: 257-260
URI: http://hdl.handle.net/123456789/7732
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.04(2) [April 2005]

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