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Title: Plantlet regeneration via adventitious shoot bud proliferation from leaf explants in Potentilla fulgens Wall. ex Hook.—A plant possessing hypoglycemic activity
Authors: Laskar, M A
Lyngdoh, J P
Buam, J J
Syiem, D
Keywords: adventitious shoot regeneration
explant browning
Potentilla fulgens
Issue Date: Apr-2005
Publisher: CSIR
IPC CodeInt. Cl.7 A01H4/00, 5/00
Abstract: A method for adventitious shoot bud regeneration from leaves of Potentilla fulgens has been developed. Leaves were obtained from plants growing in the natural habitat. Explant browning, a major hurdle in the establishment of cultures, was overcome by treating leaves with a combination of antioxidants (100 mg l-1 ascorbic acid, 100 mg l-1 citric acid and 20 mg l-1 L-cysteine HCl). Influence of the growth regulators BAP (6-benzylaminopurine) and NAA ( -naphthaleneacetic acid) on adventitious bud differentiation and shoot regeneration was observed on modified Murashige and Skoog’s (MMS) agar medium. The most effective treatment was MMS with 0.1 mg l-1 BAP and 0.1 mg l-1 NAA, which gave 80% bud induction frequency with 38.4 BFC (Bud Forming Capacity) index and 48 shoots per explant of 3.5 cm length. Rooting was induced on MS basal medium. The regenerated plants had 70% survival rate.
Page(s): 257-260
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.04(2) [April 2005]

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