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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(2) [April 2005] >
| Title: | Plantlet regeneration via adventitious shoot bud proliferation from leaf explants in Potentilla fulgens Wall. ex Hook.—A plant possessing hypoglycemic activity |
| Authors: | Laskar, M A
Lyngdoh, J P
Buam, J J
Syiem, D |
| Keywords: | adventitious shoot regeneration
explant browning
Potentilla fulgens |
| Issue Date: | Apr-2005 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 A01H4/00, 5/00 |
| Abstract: | A method for
adventitious shoot bud regeneration from leaves of Potentilla fulgens has been developed. Leaves were obtained from
plants growing in the natural habitat. Explant browning, a major hurdle in the
establishment of cultures, was overcome by treating leaves with a combination
of antioxidants (100 mg l-1 ascorbic acid, 100 mg l-1
citric acid and 20 mg
l-1 L-cysteine HCl). Influence of the growth regulators BAP
(6-benzylaminopurine) and NAA (  -naphthaleneacetic acid) on adventitious bud
differentiation and shoot regeneration was observed on modified Murashige and
Skoog’s (MMS) agar medium. The most effective treatment was MMS with 0.1 mg l-1
BAP and 0.1 mg l-1 NAA, which gave 80% bud induction frequency with
38.4 BFC (Bud Forming Capacity) index and 48 shoots per explant of 3.5 cm
length. Rooting was induced on MS basal medium. The regenerated plants had 70%
survival rate. |
| Page(s): | 257-260 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.04(2) [April 2005]
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