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|Title:||Organogenic plant regeneration via callus induction in chickpea (Cicer arietinum L.)—Role of genotypes, growth regulators and explants|
Chawla, H S
|Series/Report no.:||Int. Cl.7 A01H4/00, 5/00|
|Abstract:||Organogenic plant regeneration via callus induction was studied in genotypes Pusa256 and PG186 from four explants, viz. immature embryos, immature cotyledons, mature embryo axes and mature cotyledons, on media containing different combinations and concentrations of growth regulators. Different callusing media containing varying levels of NAA (0.5 to 2 mg/l) with or without BAP (1 mg/l) were tested for callus induction response. Maximum (90%) callusing response was obtained from immature cotyledon explants on medium supplemented with 2 mg/l NAA and 1 mg/l BAP. However, only 64% of induction frequency was obtained from immature embryos on medium containing 2 mg/l NAA. After 8 weeks of induction, the calluses were transferred on different regeneration media containing varying levels of BAP (1 to 3 mg/l) with or without NAA (0.05 mg/l). Multifactorial analysis of the study revealed that calluses, induced from a medium with high callus induction frequency, did not show high shoot induction frequency. Thus, all the factors, viz. explants, genotypes and growth regulators, during callus induction play a significant role in the subsequent high frequency shoot regeneration. In general, calluses induced from embryonic explants on medium with 1.5 mg/l NAA and 1 mg/l BAP showed 17-18% shoot regeneration on a medium containing 1-2 mg/l BAP alone or in combination with 0.05 mg/l NAA.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.04(2) [April 2005]|
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