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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(2) [April 2005] >
| Title: | Organogenic plant regeneration via callus induction in chickpea (Cicer arietinum L.)—Role of genotypes, growth regulators and explants |
| Authors: | Arora, Anju
Chawla, H S |
| Keywords: | chickpea
organogenic regeneration
callus induction |
| Issue Date: | Apr-2005 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 A01H4/00, 5/00 |
| Abstract: | Organogenic
plant regeneration via callus induction was studied in genotypes Pusa256 and
PG186 from four explants, viz. immature embryos, immature cotyledons, mature
embryo axes and mature cotyledons, on media containing different combinations
and concentrations of growth regulators. Different callusing media containing
varying levels of NAA (0.5 to 2 mg/l) with or without BAP (1 mg/l) were tested
for callus induction response. Maximum (90%) callusing response was obtained
from immature cotyledon explants on medium supplemented with 2 mg/l NAA and 1
mg/l BAP. However, only 64% of induction frequency was obtained from immature
embryos on medium containing 2 mg/l NAA. After 8 weeks of induction, the
calluses were transferred on different regeneration media containing varying
levels of BAP (1 to 3 mg/l) with or without NAA (0.05 mg/l). Multifactorial
analysis of the study revealed that calluses, induced from a medium with high
callus induction frequency, did not show high shoot induction frequency. Thus,
all the factors, viz. explants, genotypes and growth regulators, during callus
induction play a significant role in the subsequent high frequency shoot
regeneration. In general, calluses induced from embryonic explants on medium
with 1.5 mg/l NAA and 1 mg/l BAP showed 17-18% shoot regeneration on a medium
containing 1-2 mg/l BAP alone or in combination with 0.05 mg/l NAA. |
| Page(s): | 251-256 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.04(2) [April 2005]
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