NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(2) [April 2005] >


Title: A study on accumulation of PHB in native Pseudomonas isolates LDC-5 and LDC-25
Authors: Sujatha, K
Mahalakshmi, A
Shenbagarathai, R
Keywords: polyhydroxyalkanoate
PHA synthase
colony PCR
seminested PCR
biodegradable plastics
Pseudomonas
Issue Date: Apr-2005
Publisher: CSIR
IPC CodeInt. Cl.7 C08G63/00; C12N15/10; C12R1:38
Abstract:  Poly (-hydroxyalkanoates) (PHAs) are natural polyesters produced by a variety of bacteria.They are represented most commonly by poly (-hydroxybutyrate) (PHB), an intracellular storage biodegradable polymer material. The production costs of PHB are quite high compared with those of synthetic non-degradable plastics, hence search for potential strains with high PHB accumulating ability. Hundreds of indigenous bacterial strains were screened for the accumulation of PHB by Nile red, fluorescence microscopy (Nile blue A) and PCR. Three degenerate primers were used as PCR primers to detect PHA synthase genes. Among the tested isolates, 35 strains yielded a specific amplicon of 496 bp and 406 bp in colony PCR and seminested PCR, respectively. Among the 35 short chain length positive strains, only 2 isolates yielded a specific amplicon of 540 bp PCR product in medium chain length PCR, representing partial coding sequences of phaC1/phaC2 genes. The mcl-PCR positive Pseudomonas indigenous isolates (LDC-5 and LDC-25) could be potential candidates for bioplastic production.
Page(s): 216-221
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.04(2) [April 2005]

Files in This Item:

File Description SizeFormat
IJBT 4(2) 216-221.pdf461.95 kBAdobe PDFView/Open
 Current Page Visits: 680 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 636908 since 06-Feb-2009  Last updated on 15-Dec-2014Webmaster: nopr@niscair.res.in