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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(2) [April 2005] >
| Title: | A study on accumulation of PHB in native Pseudomonas isolates LDC-5 and LDC-25 |
| Authors: | Sujatha, K
Mahalakshmi, A
Shenbagarathai, R |
| Keywords: | polyhydroxyalkanoate
PHA synthase
colony PCR
seminested PCR
biodegradable plastics
Pseudomonas |
| Issue Date: | Apr-2005 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 C08G63/00; C12N15/10; C12R1:38 |
| Abstract: |
Poly ( -hydroxyalkanoates) (PHAs) are natural polyesters produced by a
variety of bacteria.They are represented most commonly by poly (-hydroxybutyrate) (PHB), an intracellular storage biodegradable
polymer material. The production costs of PHB are quite high compared with
those of synthetic non-degradable plastics, hence search for potential strains
with high PHB accumulating ability. Hundreds of indigenous bacterial strains
were screened for the accumulation of PHB by Nile
red, fluorescence microscopy (Nile blue A) and PCR. Three degenerate primers
were used as PCR primers to detect PHA synthase genes. Among the tested
isolates, 35 strains yielded a specific amplicon of 496 bp and 406 bp in colony
PCR and seminested PCR, respectively. Among the 35 short chain length positive
strains, only 2 isolates yielded a specific amplicon of 540 bp PCR product in
medium chain length PCR, representing partial coding sequences of phaC1/phaC2 genes. The mcl-PCR positive Pseudomonas indigenous isolates (LDC-5 and LDC-25) could be
potential candidates for bioplastic production.
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| Page(s): | 216-221 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.04(2) [April 2005]
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