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IJBT Vol.04(1) [January 2005] >


Title: Transgenic cabbage (Brassica oleracea var. capitata) resistant to Diamondback moth (Plutella xylostella)
Authors: Paul, Anderson
Sharma, S R
Sresty, T V S
Devi, Shantibala
Bala, Suman
Kumar, P S
Saradhi, P Pardha
Frutos, Roger
Altosaar, I
Kumar, P Ananda
Keywords: Bacillus thuringiensis
insecticidal protein genes
cry1Ab
cry1B
cabbage
Diamondback moth
transgenic plants
Issue Date: Jan-2005
Publisher: CSIR
IPC CodeInt. Cl.7 C 12 N 15/00, 15/09, 15/11
Abstract: A synthetic fusion gene of Bacillus thuringiensis encoding a translational fusion product of Cry1B and Cry1Ab δ-endotoxins was transferred to a tropical cabbage breeding line by Agrobacterium-mediated transformation. Selection of transformants was carried out on media containing kanamycin. Polymerase chain reaction (PCR) analysis revealed that twelve of the putative transformants contained the transgene. Insect bioassays carried out with the leaves of PCR-positive plants and neonate larvae of Diamondback moth (DBM) showed that one of the transgenic plants was completely resistant to repeated infestation by the larvae. Southern hybridization confirmed gene integration in the DBM-resistant plant. Double-antibody sandwich Enzyme-Linked Immunosorbant Assay (ELISA) analysis revealed accumulation of fusion protein up to 0.16% of total soluble protein in the leaves of the transgenic plants. Progeny (T1 generation) of the selfed transgenic plants were analyzed for the transgene segregation and insect protection. These studies clearly demonstrated the efficacy of Cry1B-Cry1Ab fusion protein to confer protection to cabbage against DBM infestation. The transgenic cabbage plants will serve as a good system to study the role of gene pyramiding in resistance management strategies intended to prevent evolution of resistance in DBM.
Page(s): 72-77
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.04(1) [January 2005]

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