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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(1) [January 2005] >
| Title: | Transgenic cabbage (Brassica oleracea var. capitata) resistant to Diamondback moth (Plutella xylostella) |
| Authors: | Paul, Anderson
Sharma, S R
Sresty, T V S
Devi, Shantibala
Bala, Suman
Kumar, P S
Saradhi, P Pardha
Frutos, Roger
Altosaar, I
Kumar, P Ananda |
| Keywords: | Bacillus thuringiensis
insecticidal protein genes
cry1Ab
cry1B
cabbage
Diamondback moth
transgenic plants |
| Issue Date: | Jan-2005 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 C 12 N 15/00, 15/09, 15/11 |
| Abstract: | A synthetic fusion gene of Bacillus thuringiensis encoding a
translational fusion product of Cry1B and Cry1Ab δ-endotoxins was transferred
to a tropical cabbage breeding line by Agrobacterium-mediated
transformation. Selection of transformants was carried out on media containing
kanamycin. Polymerase chain reaction (PCR) analysis revealed that twelve of the
putative transformants contained the transgene. Insect bioassays carried out
with the leaves of PCR-positive plants and neonate larvae of Diamondback moth
(DBM) showed that one of the transgenic plants was completely resistant to
repeated infestation by the larvae. Southern hybridization confirmed gene
integration in the DBM-resistant plant. Double-antibody sandwich Enzyme-Linked
Immunosorbant Assay (ELISA) analysis revealed accumulation of fusion protein up
to 0.16% of total soluble protein in the leaves of the transgenic plants.
Progeny (T1 generation) of the selfed transgenic plants were
analyzed for the transgene segregation and insect protection. These studies
clearly demonstrated the efficacy of Cry1B-Cry1Ab fusion protein to confer
protection to cabbage against DBM infestation. The transgenic cabbage plants
will serve as a good system to study the role of gene pyramiding in resistance
management strategies intended to prevent evolution of resistance in DBM. |
| Page(s): | 72-77 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.04(1) [January 2005]
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