Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/7725
Title: Plant regeneration from semi-mature zygotic embryos of <i style="">Dalbergia sissoo </i>Roxb.
Authors: Chand, Suresh
Singh, Ajay Kumar
Keywords: <i style=""> Dalbergia sissoo</i>
plant regeneration
semi-mature zygotic embryos
Issue Date: Jan-2005
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>7</sup> A 01 H 5/00, 5/10
Abstract: Plant regeneration from callus cultures derived from semi-mature zygotic embryos of <i style="">Dalbergia sissoo </i>Roxb. was achieved. Callus formation occurred on Murashige and Skoog (1962) medium supplemented with 2.26-13.57 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> 2,4-D in combination with 0.46 and 1.16 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> Kn. Maximum response for callus formation was 78.3% on MS medium containing 9.04 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> 2,4-D and 1.16 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> Kn. Shoot regeneration occurred when calli clumps were transferred to MS medium enriched with 2.22-13.32 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> BAP and 1.34 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> NAA. Maximum response (45%) for shoot regeneration was achieved when calli clumps were transferred to MS medium supplemented with 8.88 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> BAP and 1.34 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> NAA. Average number of shoots per callus clump was 7.5 after 15 weeks of culture. <i style="">In vitro</i> regenerated shoots were rooted on ½ MS medium containing 1.23 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> IBA. Rooted plantlets transferred to pots containing autoclaved peat moss, soil, and compost mixture (1:1:1) were successfully established in pots.
Description: 78-81
URI: http://hdl.handle.net/123456789/7725
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.04(1) [January 2005]

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