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Title: Plant regeneration from semi-mature zygotic embryos of Dalbergia sissoo Roxb.
Authors: Chand, Suresh
Singh, Ajay Kumar
Keywords:  Dalbergia sissoo
plant regeneration
semi-mature zygotic embryos
Issue Date: Jan-2005
Publisher: CSIR
IPC CodeInt. Cl.7 A 01 H 5/00, 5/10
Abstract: Plant regeneration from callus cultures derived from semi-mature zygotic embryos of Dalbergia sissoo Roxb. was achieved. Callus formation occurred on Murashige and Skoog (1962) medium supplemented with 2.26-13.57 M 2,4-D in combination with 0.46 and 1.16 M Kn. Maximum response for callus formation was 78.3% on MS medium containing 9.04 M 2,4-D and 1.16 M Kn. Shoot regeneration occurred when calli clumps were transferred to MS medium enriched with 2.22-13.32 M BAP and 1.34 M NAA. Maximum response (45%) for shoot regeneration was achieved when calli clumps were transferred to MS medium supplemented with 8.88 M BAP and 1.34 M NAA. Average number of shoots per callus clump was 7.5 after 15 weeks of culture. In vitro regenerated shoots were rooted on ½ MS medium containing 1.23 M IBA. Rooted plantlets transferred to pots containing autoclaved peat moss, soil, and compost mixture (1:1:1) were successfully established in pots.
Page(s): 78-81
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.04(1) [January 2005]

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