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IJBT Vol.04 [2005] >
IJBT Vol.04(1) [January 2005] >
| Title: | Plant regeneration from semi-mature zygotic embryos of Dalbergia sissoo Roxb. |
| Authors: | Chand, Suresh
Singh, Ajay Kumar |
| Keywords: | Dalbergia sissoo
plant regeneration
semi-mature zygotic embryos |
| Issue Date: | Jan-2005 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 A 01 H 5/00, 5/10 |
| Abstract: | Plant
regeneration from callus cultures derived from semi-mature zygotic embryos of Dalbergia sissoo Roxb. was achieved.
Callus formation occurred on Murashige and Skoog (1962) medium supplemented
with 2.26-13.57  M 2,4-D in combination with 0.46 and 1.16 M Kn. Maximum response for callus formation was 78.3% on MS medium
containing 9.04 M 2,4-D and 1.16 M Kn. Shoot regeneration
occurred when calli clumps were transferred to MS medium enriched with
2.22-13.32 M BAP and 1.34 M NAA. Maximum response
(45%) for shoot regeneration was achieved when calli clumps were transferred to
MS medium supplemented with 8.88 M BAP and 1.34 M NAA. Average number of shoots per callus clump was 7.5 after 15 weeks
of culture. In vitro regenerated
shoots were rooted on ½ MS medium containing 1.23 M IBA. Rooted plantlets transferred to pots containing autoclaved
peat moss, soil, and compost mixture (1:1:1) were successfully established in
pots. |
| Page(s): | 78-81 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.04(1) [January 2005]
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