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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(1) [January 2005] >
| Title: | Increased Fe-toxicity tolerance in rice calli and modulation in isozyme profiles |
| Authors: | Roy, Bidhan
Mandal, Asit B |
| Keywords: | Fe-toxicity tolerance
isozyme analysis
rice |
| Issue Date: | Jan-2005 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 A 01 H 5/00; C 12 N 9/00 |
| Abstract: | Seed derived calli of rice cultivars, ‘IR72’
(susceptible) and ‘C14-8’ (tolerant) were screened in vitro under increasing levels (50, 100, 200 or 400 ppm) of
Fe-toxicity and profiled for isozymes, viz. esterase, peroxidase, malate
dehydrogenase and lactate dehydrogenase, to assess their involvement in
Fe-toxicity tolerance. In vitro
screening showed the detrimental effect of hifher concentration on plantlet
regeneration. However, a few calli survived on stressed medium and regenerated
plantlets. Cultivar ‘C14-8’ showed higher degree of tolerance than ‘IR72’.
Prominent differences including changes in band activity/intensity,
mobility/shift and number of polymorphic loci with respect of Fe-toxicity were
evident. The activity of esterase, malate dehydrogenase and lactate
dehydrogenase decreased in Fe-stressed medium in ‘C14-8’. Decreased activity
indicates gradual degradation of these enzymes or their structural modification
under increased Fe-toxicity levels. On the other hand, the activity of these
enzymes increased in the ‘IR72’ under stressed medium. However, the activity of
peroxidase remained almost unaltered in ‘C14-8’ across the stress gradient. Few
bands disappeared or newly appeared in the stressed medium as compared to
control. This may be due to activation or inactivation of diverse domains in
the genome and may be involved in governing Fe-toxicity tolerance. Whereas, a
few bands remained unaltered across the stress gradient, and these may be used as
biochemical marker for selection of tolerant plants against iron toxicity. |
| Page(s): | 65-71 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.04(1) [January 2005]
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