19-May-2013
02:59:26 IST
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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.03 [2004] >
IJBT Vol.03(2) [April 2004] >
| Title: | Tobacco (Nicotiana tabacum L.)-A model system for tissue culture interventions and genetic engineering |
| Authors: | Ganapathi, T R
Suprasanna, P
Rao, P S
Bapat, V A |
| Keywords: | genetic transformation
molecular farming
plant biotechnology
plant cell and tissue culture
recombinant proteins
tobacco |
| Issue Date: | Apr-2004 |
| Publisher: | CSIR |
| IPC Code: | lnt. CI.7 A 01 H 4/00, 5/00, A 61 K 35/74, 35/76, 39/002, 39/02, 39/12; C 12 N 15/00, 15/01, 15/05, 15/08, 15/09 |
| Abstract: | Tobacco (Nicotiana tabacum L.)
has become a model system for tissue culture and genetic engineering over the
past
several decades and continues to
remain the 'Cinderella of Plant Biotechnology', An ill vitro culture
medium (Murashige
and Skoog, 1962), based on the
studies with tobacco tissue cultures, has now been widely used as culture
medium
formulation for hundreds of plant
species. Studies with tobacco tissue culture have shed light on the control of in vitro
growth and differentiation.
Further, induction of haploids, microspore derived embryos and selection of
mutant cell lines,
have been achieved successfully.
Tobacco has also been employed for the culture and fusion of plant protoplasts,
providing
invaluable information on way to
explore the potential of somatic hybridization in other crops. Optimization of
genetic
transformation, using Agrobacterium
tumefaciens and A. rhizogenes, has been central to the cascade of
advances in the area
of transgenic plants. Developments
in the field of molecular farming for the expression and/or production of
recombinant
proteins, vaccines and antibodies
are gaining significance for industrial use and human healthcare. |
| Page(s): | 171-184 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.03(2) [April 2004]
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