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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.03 [2004] >
IJBT Vol.03(2) [April 2004] >
| Title: | Induced nucellar embryogenesis in vitro for clonal multiplication of Mangifera indica L. var. Ambalavi: A dwarfing rootstock |
| Authors: | Chaturvedi, H C
Agnihotri, S
Sharma, M
Sharma, A K
Jain, M
Gupta, P
Chourasia, A
Kidwai, N R |
| Keywords: | Ambalavi
cloning
dwarfing rootstock
Mangifera indica
monoembryonic
nucellar embryogenesis
rooted shoots |
| Issue Date: | Apr-2004 |
| Publisher: | CSIR |
| IPC Code: | Int Cl.7 A 01 H 4/00, 5/00 |
| Abstract: | Nucellar embryogenesis was
induced in Mangifera indica L. var.
Ambalavi, a monoembryonic dwarfing rootstock. Nucellar tissue of young fruits
of 3 developmental stages and measuring about. 2.5, 4 and 5 cm in length,
responded differently to the same treatments of agarified nutrient medium used
for induction of embryogenesis. Whilst 0.25 mg l-l BAP along with 1
mg l-l NAA was effective to induce embryogenesis in nucellus of
youngest fruits, 0.5 mg l-1 2iP alone was sufficient for nucellus of
older fruits. However, all the differentiated embryos proliferated in a single treatment comprising 0.15 mg l-1 each of BAP and 2iP and 0.5 mg l-l
IAA, albeit with a different basal medium. Size of cotyledonary nucellar
embryos ranging between 1.5 to 2 cm in length was necessary for their further
development, maturation, germination (visible plumule and developed root) and
convertibility (plantlet formation). Embryos of desirable size required the
liquid state of the medium supplemented with 0.01 mg l-1 ABA, 0.1 mg
l-1 IAA and 100 mg l-1 PEG (M. Wt. 400), while the
nitrogen content was also low for their near-synchronized development,
maturation, germination and convertibility.
In the optimum treatment, most of the embryos showed apparently normal
development, of which 78.4% matured and 40.2% germinated, while 35.6% embryos
produced plantlets. Such a high percentage of convertibility of nucellar
embryos has not been obtained earlier. The hardened in vitro-raised plantlets survived ex vitro when first transplanted to Soilrite followed by their
transfer to the garden soil. Furthermore, adventitiously rooted nucellar
plantlets gave better transplant success, i.e., about. 70% than those with
their initial root system, where it was about. 50%. The former also survived
longer than the latter, i.e., beyond 4 months. |
| Page(s): | 221-228 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.03(2) [April 2004]
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