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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(4) [October 2005] >
| Title: | Concurrent infection with WSSV and MBV in Tiger prawn, Penaeus monodon (Fabricious) in West Bengal and their detection using PCR and DNA Dot-blot hybridization technique |
| Authors: | Mishra, S S
Shekhar, M S
Azad, I S |
| Keywords: | DNA probe
MBV
PCR
Tiger prawn
white spot disease
WSSV |
| Issue Date: | Oct-2005 |
| Publisher: | CSIR |
| IPC Code: | Int.Cl.7 C12N15/10,G01N33/53 |
| Abstract: | Prevalence status of
white spot disease (WSD), caused by white spot syndrome virus (WSSV) and
Monodon baculovirus (MBV), was studied in different prawn farms in North and
South-24 paragana districts of West Bengal, specifically in the areas
previously affected by WSD. In total 283 samples of giant tiger prawn Penaeus monodon, Indian white prawn P. indicus and giant freshwater prawn Macrobrachium rosenbergii from 12
different farms were screened and presence of MBV occlusion-bodies (Obs) were
detected. Of samples screened, 30 samples showed multiple spherical Obs typical
of MBV infection. Of 80 samples screened using PCR, 17 (21.25%) were positive
for MBV and 51 samples (63.75%) were positive for WSSV, and amplified DNA of
674 bp and 643 bp, respectively was visualized on 1% agarose gel
electrophoresis. Significantly all samples showing positive reaction with MBV
were also positive for WSSV in PCR, indicating co-infection of prawns with both
MBV and WSSV. However, none of the M.
rosenbergii samples were positive of MBV-Obs but 5 samples were positive for
WSSV in PCR. Both MBV and WSSV amplified DNA were labeled with alkaline
phosphatase using Alkphos DNA labeling reagent kit and samples were screened
using DNA dot-blot and slot-blot hybridization. All samples showing positive
PCR reaction were also found positive in DNA hybridization |
| Page(s): | 506-515 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.04(4) [October 2005]
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