NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(2) [April 2005] >

Title: Mutational scanning of RB1 gene by multiplex PCR
Authors: Joseph, Biju
Narayana, Komaravelly Siva
Mamatha, Gandra
Raman, Gayathree
Keywords: cost analysis
RB1 gene
Issue Date: Apr-2005
Publisher: CSIR
IPC CodeInt. Cl.7 C12N15/07; 15/10
Abstract:  Multiplex PCR (mPCR) strategy was applied for mutational screening of the exons of RB1 gene in retinoblastoma patients from India. After ethanol purification, the amplified products were directly cycle-sequenced using fluorescent dNTP (deoxy nucleotide triphosphate) ready reaction mix, followed by sequencing in an ABI PRISM 310 automated sequencer. Eighteen of the 27 exons of RB1 gene were amplified in eight multiplex groups with direct savings of 46% of PCR time and 30% of reagents compared to individual amplifications. Thus, the mPCR strategy for sequencing comparatively offered rapid diagnosis and considerable amount of savings in PCR time and reagents’ cost. The mutation results were used for genetic counselling of the patients and their families.
Page(s): 194-200
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.04(2) [April 2005]

Files in This Item:

File Description SizeFormat
IJBT 4(2) 194-200.pdf468.71 kBAdobe PDFView/Open
 Current Page Visits: 759 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 169375 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: