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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.48 [2010] >
IJEB Vol.48(04) [April 2010] >
| Title: | Hepatocyte growth factor (HGF), heat shock proteins (HSPs) and multidrug resistance protein (MRP) expression in co-culture of colon tumor spheroids with normal cells after incubation with interleukin-1 (IL-1) and/or camptothecin (CPT-11) |
| Authors: | Paduch, Roman
Gil, Joanna Jakubowicz
Niedziela, Piotr |
| Keywords: | Co-culture
Colon carcinoma
Heat shock proteins
Hepatocyte growth factor
Multidrug resistance protein |
| Issue Date: | Apr-2010 |
| Publisher: | CSIR |
| Abstract: | Tumor
chemoresistance and metastasis are some of the most important problems in colon
cancer therapy. In the present study, co-cultures of human colon carcinoma cell
spheroids, obtained from different grades of tumor, with human colon
epithelium, myofibroblast and endothelial cell monolayers were performed. The
purpose of these co-cultures was to reflect, in in vitro conditions,
different stages of colon tumor development. In order to investigate the
invasive capacities of the tumor cells and their resistance to chemotherapy,
HGF, HSP27, HSP72 and MRP levels were analyzed after incubation of the
co-cultures with IL-1 and
irinotecan (CPT-11) added as single agents or in combination. Myofibroblasts
produced significantly higher amounts of HGF than epithelial cells. Tumor cells
released trace amounts of this molecule. In co-cultures, IL-1 induced HGF release, while CPT-11 alone or combined with IL-1decreased HGF secretion. An immunoblotting analysis followed by
densitometry revealed that the combination of IL-1 plus CPT-11 added to the co-cultures led to a decrease in HSPs and
MRP levels. In conclusion, direct and paracrine interactions of colon tumor
cell spheroids with normal cells and exogenously added CPT-11 change HSP27,
HSP72 and MRP expression in comparison to monocultures. IL-1 and CPT-11, dependent on whether they are added separately or
jointly, differentially modulate HGF expression in monocultures of colon tumor
spheroids or normal cells and their co-cultures. |
| Page(s): | 354-364 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.48(04) [April 2010]
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