Antibacterial effects of goat and chicken heart tissues against human pathogenic bacteria

Abstract

The crude buffer (Tris Buffer Saline-I) extracts of muscles, liver, kidney and heart of goat and chicken (White leghorn) were screened against 16 clinical isolates. Among the five tissues, the heart tissue of each animal showed significant bactericidal activities on many isolates. The acid extracted crude proteins of both heart tissues also showed significant antibacterial activities against many bacterial isolates. The crude proteins of goat heart tissues displayed strong bactericidal activities against <i style="">Salmonella paratyphi</i> ‘A’ and <i style="">Salmonella typhimurium</i> (MIC: 16 <img src='/image/spc_char/micro.gif' border=0>g/ml) whereas the crude proteins of chicken heart tissues displayed strong bactericidal activities against <i style="">Escherichia coli</i> ATCC and <i style="">Pseudomonas aeruginosa</i> at 16 and 63 <img src='/image/spc_char/micro.gif' border=0>g/ml concentrations respectively. The peptides of low molecular weight (< 30 kDa) were also separated from the acid extracted crude proteins of goat and chicken heart tissues by SDS-PAGE after staining with silver nitrate solution.